关节炎大鼠吗啡耐受模型的建立

Establishment of morphine tolerance model with arthritis in rat

目的:建立一种以慢性炎症为基础的吗啡耐受模型。方法:将32只健康雄性SD大鼠随机分为4组,关节炎鞘内给予吗啡组(A组),关节炎腹腔给予吗啡组(B组),单纯鞘内给予吗啡组(C组),单纯腹腔给予吗啡组(D组)。A组与C组鞘内给予10μg/kg吗啡1日2次,B组与D组经腹腔给予同样剂量的吗啡。以热板法缩爪潜伏期和50%缩爪阈值作为行为学指标进行观察。结果:给药后第7天,A组大鼠的行为学指标较B、C、D组有统计学差异,C组与B、D两组相比有统计学差异,而B、D两组间无统计学差异。结论:可以在慢性炎症的基础上经鞘内给药制作出吗啡耐受的大鼠模型,而且本模型较以前的模型更加接近于实际。

Objective： To establish a morphine tolerance model with chronic arthritis in rat. Methods： The 32 SD rats were randomly derided into four groups. Group A： administered morphine intrathecally in rats with arthritis. Group B： administered morphine intraperitoneally in rats with arthritis Group C： administered morphine intrathecally in rats without arthritis Group D：administered morphine intraperitoneally in rats without arthritis.The group A and group C were intrathecally administered morphine 10 p.g/kg two times a day and the group B and group D were intraperitoneally administered the same dose of morphine. The thermal withdral latency （TWL） and mechanical withdrawl threshold （MWT） of rats were used to evaluate its behaviour. Results： Compared with the B,C,D groups, the TWL and MWT in group A has the statistic significance after 7 days administering drugs. Compared with the B,D groups, the TWL and MWT in group C has the statistic significance. There no statistic significance between Bgroup and D group. Conclusion： The morphine tolerance model with chronic arthritis of rat can be established and it is more practicable than the previous model.