对氧磷酶-1与2型糖尿病的关系研究

Study on the relationship between paraoxonase-1 and type 2 diabetes mellitus

目的:探讨对氧磷酶-1(PON-1)与2型糖尿病(T2DM)的关系。方法:测定63例T2DM患者和33例健康人血清PON-1活性,并应用聚合酶链式反应—限制性片段长度多态性(PCR-RFLP)技术研究其PON-1第191位密码子基因变异。结果:(1)T2DM组血清PON-1活性低于对照组,差别具有统计学意义(t=2.75,P<0.05)。(2)T2DM组与对照组PON-1第191位密码子基因型分布无显著性差异(2χ=3.03,P>0.05)。结论:T2DM组血清PON-1活性显著低于对照组,这可能是T2DM患者血管疾病高发的原因之一。

Objective： To reveal the relationship between serum paraoxonase-1 （PON-1） activity, PON-1 Q/R polymorphism and type 2 diabetes mellitus in Chinese Han ethnic group. Methods： The subjects of the study consisted of two groups： （1） type 2 diabetes mellitus group; （2） normal-control group. Serum PON-1 activity was determined using paraoxon as substrate by measuring the increase rate in the absorbance at 405 nm due to formation of 4-nitrophenol, and PON-1 Q/R polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP）. Results：（1） There was no significant difference in the distribution of the PON-1 Q/R polymorphism between T2DM group and normal-control group （X^2 =3.03,P〉0.05）. （2） Serum PON-1 activity in T2DM group and normal-control group was （44.65±19.96）u/ml and （74.85±27.72）u/ml, respectively. Serum PON-1 activity in the former group was significantly low compared with the control group （t=2.75,P 〈0.05）. Conclusion： The present study suggests that serum PON-1 activity in T2DM group is significantly lower than the control group. Perhaps it is one of the reasons why T2DM patients are highly complicated with vascular diseases.