早期流产患者绒毛中抑制素A、激活素A的表达及其意义

The expression of inhibin A and activin A at the villus of early abortion

目的:了解抑制素A、激活素A在早孕、先兆流产绒毛中的定位及表达水平;探讨抑制素A、正常妊娠活素A在维持正常早期妊娠中的作用。方法:选取60例(停经天数<60 d)早期妊娠妇女作为研究对象,其中行负压吸宫术者30例为对照组;行吸宫术的先兆流产妇女30例为先兆流产组,留取绒毛组织,应用免疫组织化学技术和计算机图像分析系统检测抑制素A、激活素A在不同早孕流产绒毛组织中的表达。结果:抑制素A、激活素A在对照组、先兆流产组绒毛的合体滋养细胞、细胞滋养细胞、毛细血管内皮细胞的胞浆呈不同程度的阳性染色。通过定量分析,抑制素A、激活素A在先兆流产组绒毛的表达低于对照组,差异有显著性(P<0.05)。结论:胎盘组织分泌的抑制素A、激活素A对于正常妊娠的维持起重要作用;抑制素A、激活素A表达水平的降低在流产发生过程中具有重要作用。

Objective： To study the position and expresses of inhibin A and activin A at the villus of the normal early pregnancy and threatened abortion, and the effect of inhibin A and activin A for sustaining the normal early pregnancy. Methods： 60 early pregnancy women without pregnancy complications （the days of menelipsis less than 60 days） were collected, including 30 cases normal pregnancy of artificial abortion, 30 cases of threatened abortion. The expression of inhibin A and activin A in the villus of early pregnancy abortion were determined by immunohistochemistry technology and computer image analysis systems. Results： Inhibin A and activin A exhibited positive staining at the cytoplasm of syncytiotrophoblast, cytotrophoblast and capillary endothelial cell of villus. Through quantitative analysis, The expression of inhibin A and activin A in the villus of the threatened abortion group was less than in the control group, the difference was significant （P〈0.05）. Conclusion： Inhibin A and activin A from placental tissue play an important role in sustaining the normal early pregnancy; the decrease levels of inhibin A and activin A play an important role in the process of abortion.