MMP-2和TIMP-2在甲状腺癌中的表达及其意义

Expression of matrix metalloproteinases-2 and tissue inhibitor of metalloproteinase-2 in thyroid carcinoma

目的:探讨金属基质蛋白酶-2(MMP-2)和金属基质蛋白酶组织抑制剂-2(TIMP-2)在甲状腺良恶性肿瘤中的表达情况,分析MMP-2和TIMP-2与甲状腺癌的相关性。方法:采用逆转录酶-聚合酶链反应检测32例甲状腺癌、30例甲状腺良性肿瘤及18例癌旁组织MMP-2和TIMP-2mRNA的表达。结果:在甲状腺癌、甲状腺良性肿瘤和癌旁组织中MMP-2的表达率分别为78.1%(25/32)、20%(6/30)、16.7%(3/18),TIMP-2的表达率为65.6%(21/32)、26.7%(8/30)、11.1%(2/18),MMP-2的表达率在TNM分期Ⅲ+Ⅳ及淋巴结转移的标本中要高于TNM分期Ⅰ+Ⅱ及无淋巴结转移的标本,但TIMP-2的表达在这两类中无差别。在甲状腺癌组织中MMP-2和TIMP-2的表达呈正相关。Logistic回归分析结果显示:MMP-2与甲状腺癌关系密切,TIMP-2与甲状腺癌无相关性。结论:MMP-2和TIMP-2在甲状腺癌中表达明显提高,两者的表达呈正相关。MMP-2与甲状腺癌的关系更为密切,其表达上调往往提示甲状腺癌的转移和侵袭。

Objective： To study the expression of matrix metalloproteinase（MMP）-2 and tissue inhibitor of metalloproteinase （TIMP）-2 in thyroid carcinoma, and analysis the correlation between MMP-2,TIMP-2 and thyroid carcinoma. Methods ：The expression of MMP-2 and TIMP-2 mRNA of 32 cases of carcinoma, 30 cases of adenoma and 18 cases of paraneoplastic tissue was detected by reverse transcriptasepolymerase chain reaction （RT-PCR）. Results ：The expression rate of MMP-2 in carcinoma, adenoma and paraneoplastic tissue was 78.1%（25/32）, 20%（6/30）, 16.7%（3/18） respectively, and TIMP-2 was 65.6%（21/ 32）, 26.7%（8/30）, 11.1 （2/18） respectively. There was a statistically significant higher expression of MMP-2 in TNM Ⅲ＋Ⅳ and lymph node metastasis than TNM in Ⅰ ＋ Ⅱ and no lymph node metastasis samples,but the expression of TIMP-2 had no statistically significant in the two gropes. There was a statistically significant positive correlation between expression of MMP-2 and TIMP-2.Logistic regression analysis displayed that there was a intimate relation between MMP-2 and thyroid carcinoma, and TIMP-2 had not. Conclusion： The mRNA expression of MMP-2 and TIMP-2 up-regulates in thyroid carcinoma, and has a positive relation. MMP-2 had a more intimate relation with thyroid carcinoma than TIMP-2 and Up-regulations of MMP-2 mRNA expression often indicates the metastasis and invasions of thyroid carcinoma.