VEGF、LMP-1在鼻咽癌中的表达及其与预后的相关性

The expression of vascular endothelial growth factor and latent membrane protein-1 and prognosis in nasopharyngeal carcinoma

目的:研究血管内皮生长因子(VEGF)、EB病毒膜潜伏蛋白-1(LMP-1)在鼻咽癌中的表达及其与预后的相关性。方法:收集50例鼻咽癌患者的临床资料及鼻咽部瘤体活检组织蜡块。采用免疫组化S-P法检测蜡块标本中VEGF、LMP-1的表达情况。VEGF、LMP-1与淋巴结转移的关系,LMP-1与VEGF相互关系采用Spearman秩相关法进行相关分析。采用Cox比例风险模型对导致鼻咽癌患者不良预后的各种因素进行多因素分析,筛选出对预后有显著意义的独立预后因素,并应用Kaplan-Meier法进行生存分析。结果:VEGF和LMP-1的阳性表达率分别为58%和50%,它们的表达均与颈淋巴结转移关系密切(P<0.05)。LMP-1与VEGF表达呈显著正相关(r=0.717,P<0.001)。鼻咽癌预后不良的危险因素是VEGF和LMP-1表达阳性(P<0.05)。结论:VEGF、LMP-1在鼻咽癌中的阳性表达与颈淋巴结转移明显相关,LMP-1能上调VEGF表达,从而增强鼻咽癌的转移潜能。二者可作为预测鼻咽癌预后的指标,从而指导临床制定个体化治疗方案,提高鼻咽癌的生存率。

Objective： To assess expression of vascular endothelial growth factor （VEC10 and latent membrane protein-1（LMP-1） and prognosis in nasopharyngeal carcinoma（NPC）. Methods：The expression of VEGF and LMP-1 were examined in 50 NPC section by immunohistochemical method. Spearman＇s Rank Correlation test was used to analysis the relation that VEGF, LMP-1 and lymph node change,as well as LMP-1 and VEGF interrelation. The Cox＇ proportional hazard model was adopted to analyze the various factors leading to the bad prognosis of nasopharyngeal carcinoma patient and screen out the independent prognosis factor having notable meaning to the prognosis. Kaplan-Meier method was used to carry out survival analysis. Results ：The positive expression rate of VEGF and LMP-1 were 58 %and 50 %, correlated with lymph node metastasis of neck （P〈0.05）. The expression of VEGF showed a significant positive correction with the expression of LMP-1 （r=0.717, P 〈0.001）. The risk factor predicting unfavorable prognosis in NPC was the positive expression of VEGF and LMP-1 （P 〈0.05）. Conclusion：The positive expression of VEGF and LMP- 1 highly correlated with lymph node metastasis of neck. The induction of VECF by LMP-1 contributes to the metastatic potential of NPC. VEGF and LMP-1 can be used as predictor of prognosis,which can direct to formulate individuation treatment plan for improving survival rate of NPC.