多巴胺D3受体对安非他明诱导的条件性位置偏爱小鼠的作用

The Role of Dopamine D3 Receptor in the Amphetamine-induced Conditioned Place Preference in Mice

目的研究多巴胺D3受体对安非他明条件性位置偏爱小鼠的作用。方法采用条件性位置偏爱系统,观察腹腔注射安非他明前后多巴胺D3受体基因敲除小鼠(D3RKO)及具有相同遗传背景的野生型小鼠(C57BL/6)的位置偏爱效应,采用SPSS13.0统计软件包对实验数据进行两因素重复测量方差分析以及显著性检验。结果安非他明(5mg/kg)能使D3RKO小鼠产生明显的条件性位置偏爱效应,给药前、后以及同生理盐水对照组相比均有显著性差异(P<0.001),而C57BL/6小鼠没有形成明显条件性位置偏爱效应(P>0.05)。结论多巴胺D3受体基因敲除后可以使小鼠对安非他明产生明显条件性位置偏爱效应,提示多巴胺D3受体基因可能对安非他明依赖性形成具有抑制性作用。

Objective To study the role of dopamine D3 receptor involved in the amphetamine-induced conditioned place preference （CPP） in mice. Methods The CPP was observed in D3 receptor knock-out （D3RKO） mice and C57BL/6 wild-type control mice after administration of amphetamine. The data were analyzed with a two-way ANOVA using the SPSS 13.0 software. Results D3RKO mice showed a significant amphetamine-induced CPP （P〈0.001）, compared with the ones administered with saline in C57BL/6 control mice. Conclusion The results indicate that amphetamine can produce significant CPP in dopamine D3 receptor knock-out mice, suggesting that amphetamine-induced addiction can be inhibited by dopamine D3 receptor.