摘要
目的探讨人骨骼肌组织中线粒体DNA 4977bp片段的缺失情况及其与年龄的相关性。方法收集105例不同年龄死者的骨骼肌组织,抽提mtDNA,通过PCR、琼脂糖紫外凝胶成像等技术,测定不同年龄组线粒体DNA 4977bp片段的缺失情况。结果在105例样品中,不同年龄组(0~9、10~19、20~29、30~39、40~49、50~59、60~69、70~79、80~89、90~99岁)mtDNA4977片段缺失的频率分别为:0、0、0.003%、0.011%、0.015%、0.033%、0.038%、0.062%、0.069%、0.091%。结论人骨骼肌线粒体DNA 4977bp片段的缺失频率随着年龄的增长而增高,各年龄组之间具有显著差异(P<0.01)。该缺失片段的检测对于软组织年龄推测具有一定指导意义。
Objective To explore depletion of human mitochondrial DNA 4977-bp and its relation with aging. Methods Total DNA (nuclear and mtDNA) was extracted from 100 mg muscle tissue. UV light illumination of ethidium bromide-stained PCR products was used to study the depletion of mtDNA (wildtype or mutant), Results The proportions of mtDNA depletion in human skeletal muscle could be determined. The frequency of mtDNA 4977-bp depletion in different age groups (0-9, 10-19, 20-29, 30-39, 40-49, 50-59, 60-69, 70-79, 80-89, 90-99) was: 0%, 0%, 0.003%, 0.011%, 0.015%, 0.033%, 0.038%, 0.062%, 0.069%, and 0.091%, respectively. Conclusion Our findings suggest that the frequency of the mtDNA4977 depletion in human skeletal muscle increases with age. It might be useful for human age estimation.
出处
《法医学杂志》
CAS
CSCD
2007年第6期438-440,共3页
Journal of Forensic Medicine
基金
浙江省2004年自然基金资助项目(Y204527)
关键词
线粒体DNA
缺失
年龄推断
mitochondrial DNA
deletion
age estimation