3种克雷伯菌产β-内酰胺酶分析

Analysis of β-lactamases produced by three species of Klebsiella spp

目的研究3种克雷伯菌所产各种β内酰胺酶（plase）分布情况。方法采用多底物纸片法（协同法、拮抗法）、超广谱G-内酰胺酶（ESBLS）确认试验、AmpC酶检测法检测51株克雷伯菌属[产酸克雷伯菌（K．ox）23株，肺炎克雷伯菌臭鼻亚种（K．oz）18株，土生克雷伯菌（K．te）10株]所产各种β—lase。结果（1）51株克雷伯菌属β—lase总检出率为80．4％，其中单独产青霉素酶、广谱酶、ESBLs菌株分别为7、4、21株，产ESBLs菌株总数32株（62．8％），未检出头孢菌素酶和碳青霉烯酶：产复合酶11株（21．6％），均产AmpC酶＋ESBLs。（2）在4种产酶形式中，K．te检出率分别10．0％、10．0％、20．0％、20．0％；K．oz检出率分别5．6％、5．6％、33．33％、27．8％：K．ox检出率分别13．0％、8．7％、56．5％、17．4％。结论（1）本组51株克雷伯菌属产β-lase总检出率很高，其中K．ox最高；（2）4种产酶形式以AmpC酶、ESBI。S为主，未检出头孢菌素酶和碳青霉烯酶，各种克雷伯菌属菌所产各种酶的检出率不同。

Objective To investigate the distribution of β-1actamases produced by three species of Klebsiella spp. Methods β-lactamases produced by 51 strains of Klebsiella spp （K. te 10 strains,K. oz 18 strains,K, ox 23 strains） were detected with a multi disk test [synergy test,antagonize test for the inducible AmpC 13-1actamases （IABLs）]AmpC β-1actamases （ABLs） phenotype test and extented- spectrum β-lactamases （ESBLs） proving test. Results （1） The total positive rate of β-lactamases was 80.4%. In 50 strains, 7 strains produced penicillinase, 4 strains produced broad-spectrum β-lactamases, 21 strains produced ESBLs separately, totally 32 strains produced ESBLs （62. 8%）, and all strains did not produce cephalosporinases and carbapenem-hydrolyzing β-1actamases （CHBLs）; 11 strains produced both of AmpC and ESBLs; （2） In 4 types of producing β-lase,the detection rates of K. te were 10.0%, 10.0%, 20.0% and 20.0% respectively; the detection ratesofK, oz were 5.6%, 5.6%, 33.33% and 27.8% respectively; and the detection rates of K ox were 13.0%, 8.7%, 56.5% and 17.4% respectively. Conclusion （1） The total detection rate of β-lactamases produced by the 51 strains of Klebsiella spp is very high, and K. ox is the highest; （2） In 4 types of producing β-lase, AmpC β-1actamases and ESBI.s are the main ones, all don＇t produce cephalosporinases and CHBLs. The detection rates of all kinds of β lactamases produced by Klebsiella spp are different.