摘要
目的 建立基于毛细管柱固定抗原流动注射增强化学发光免疫法测定IgG分析的新方法。方法以辣根过氧化物酶(HRP)标记抗IgG与抗IgG竞争毛细管内壁表面固定的IgG抗原,利用HRP催化对鲁米诺-过氧化氢-对碘苯酚体系的增强化学发光,建立测定IgG的流动注射化学发光免疫分析方法。结果在选定的实验条件下,化学发光信号与IgG在稀释比为1:1×10^11-1:1×10^10间成良好的线性关系,相关系数为0.9960。对稀释比为1:5×10^10 IgG抗体连续11次测定的相对标准偏差为6.5%。结论将该法应用于合成血清样品中IgG抗体的测定,结果满意。
Aim To study a novel method for the determination of anti-IgG. Methods A flow injection chemilumineseent (CL) immunoassay using an antigen-immobilized capillary column as reactor was developed. When the sample (anti-IgG) and the HRP-labeled anti-IgG passed through the reactor, a competitive binding for IgG antigen immobilized on the wall oeeured and the different CL intensity generated from the system of luminol-hydrogen peroxide-p-iodophenol in the presence of HRP was produced in the dependence of the concentration of anti-IgG. Results The linear range of anti-IgG from the dilution of concentration 1:1 × 10^11 to 1: 1 × 10^10 with a relative standard deviation of 6. 5% is obtained using the system designed. Conclusion This developed method has been applied to the determination of anti-IgG in human control serum with satisfactory results.
出处
《西北大学学报(自然科学版)》
CAS
CSCD
北大核心
2007年第6期996-999,共4页
Journal of Northwest University(Natural Science Edition)
基金
陕西省自然科学基金资助项目(2001H17)
关键词
流动注射分析
免疫分析
化学发光
毛细管柱
IGG
flow injection analysis
chemiluminescence immunoassay
capillary column
anti-IgG