毛细管柱固定抗原流动注射化学发光免疫法测定IgG

Flow injection chemiluminescent immunoassay determination of IgG using antigen-immobilized capillary column

目的 建立基于毛细管柱固定抗原流动注射增强化学发光免疫法测定IgG分析的新方法。方法以辣根过氧化物酶（HRP）标记抗IgG与抗IgG竞争毛细管内壁表面固定的IgG抗原，利用HRP催化对鲁米诺-过氧化氢-对碘苯酚体系的增强化学发光，建立测定IgG的流动注射化学发光免疫分析方法。结果在选定的实验条件下，化学发光信号与IgG在稀释比为1：1×10^11-1：1×10^10间成良好的线性关系，相关系数为0．9960。对稀释比为1：5×10^10 IgG抗体连续11次测定的相对标准偏差为6．5％。结论将该法应用于合成血清样品中IgG抗体的测定，结果满意。

Aim To study a novel method for the determination of anti-IgG. Methods A flow injection chemilumineseent （CL） immunoassay using an antigen-immobilized capillary column as reactor was developed. When the sample （anti-IgG） and the HRP-labeled anti-IgG passed through the reactor, a competitive binding for IgG antigen immobilized on the wall oeeured and the different CL intensity generated from the system of luminol-hydrogen peroxide-p-iodophenol in the presence of HRP was produced in the dependence of the concentration of anti-IgG. Results The linear range of anti-IgG from the dilution of concentration 1：1 × 10^11 to 1： 1 × 10^10 with a relative standard deviation of 6. 5% is obtained using the system designed. Conclusion This developed method has been applied to the determination of anti-IgG in human control serum with satisfactory results.