囊性纤维化跨膜调节因子在小鼠胸膜腔液体转运中的作用

Effect of CFTR on Pleural Fluid Transport in Mice

目的:探讨囊性纤维化跨膜调节因子(cystic fibrisis transmembrane conductance regulator.CFTR)在小鼠胸腔液体转运中的作用。方法:小鼠吸入麻醉后,胸膜腔内分别注入0.25ml等渗液体,分别予以异丙肾上腺素、CFTR激动剂CF-16和抑制剂CF_(mh)-172.在不同时间点回收胸腔液体,测量其容积。根据胸腔液体滤出率和吸收率变化,研究CFTR在胸腔液体转运中的作用。用RT-PCR检测ENaC和CFTR在胸膜上的表达情况。结果:小鼠胸膜上有ENaC和CFTR的表达,CFTR抑制剂CF_(mh)-172显著抑制异丙肾上腺素对于胸腔液体吸收的促进作用(P<0.01),激动剂CF-16不能显著增强异丙肾上腺素的这种作用(P>0.05)。结论:CFTR抑制剂可以显著抑制异丙肾上腺素对胸腔内等渗液体吸收促进作用,但是CFTR激动剂则没有明显作用。

Objective：To investigate the effect of CFTR on pleural fluid transport. Methods： Mice were briefly anesthetized, and the pleural space was infused with 0.25 ml of isosmolar solution,CFTR activator and inhibitor were added into the instillate to investigate the role of CFTR on isomolar pleural fluid absorption. Mice were euthanized at specified times for measurement of pleural fluid volume. Then the rate of pleural fluid filtration and clearance were calculated. Expression of epithelial sodium channel（ENaC）and cystic fibrosis transmembrane conductance regulator （CFTR） were detected by RT-PCR. Results： There were ENaC and CFTR expressing in the mice pleura. CFTR inhibitor greatly decreased the Isoproterenol＇s increasing pleural fluid absorption, but CFTR activator had no greatly increasing effect on Isoproterenol＇s effect. Conclusion：CFTR inhibitor significantly inhibited the Isoproerenol＇s increasing effect on pleural fluid absorption.