胆囊癌和胆管癌化疗药物敏感性测定

Drug Susceptibility Test in Carcinoma of Gallbladder and Cholangiocarcinoma

目的:探讨胆囊癌和胆管癌对化疗药物的敏感性。方法:采用四氮唑比色法检测13例胆囊癌和32例胆管癌对13肿化疗药物的敏感性。结果:胆囊癌和胆管癌的药物敏感性由高到低依次为泰素(TAL)100%,健择(GZ)75%、米托蒽醌(Mito) 66.7%,长春新碱(VCR)58.3%、羟基喜树碱(HPT)58.3%、丝裂霉素(MMC)48.9%、卡铂(CP)48.5%、、顺铂(DDP)46.7%、表阿霉素(EADM)46.7%、阿霉素(ADM)30.3%、5-氟尿嘧啶(5-FU)33.3%、甲胺喋呤(MTX)15.6%。结论:胆囊癌和胆管癌对TAL、GZ、Mito、VCR、HPT较敏感,MML、CP、DDP、EADM次之;体外药敏试验有助于临床个体化的化疗用药。

Objective：To investigate the sensibility of chemotherapy in carcinoma of gallbladder and cholangiocarcinoma. Methods：The sensibility of 13 chemotherapeutics were measured in 13 cases of carcinoma of gallbladder and 32 cases of cholangiocarcinoma by MTT assay. Results： The sensitivity of the main theraputic drugs were as follows： TAI,100%, GZ75%, Mito66, 7%, VCR58.3%, HPT 58.3%, MMC 48.9%, CP 48.5%, DDP 46.7%, EADM 46.7%, ADM 3（1.3%, 5-Fu33.3%, MTX15.6%. Conclusion：Carcinoma of gallbladder and cholangiocarcinoma are not sensitive to chemotherapy% TAI,,GZ, Mito, VCR,HPT are sensitive for cholangiocarcinoma and carcinoma of gallbladder, MMC, CP, DDP, and EADM as second. The susceptibility test in vitro is helpful to the individualized chemotherapy.