大鼠酒精性肾损害及肾组织中转化生长因子β_1的表达

Alcoholic Renal Lesion and Expression of TGF-β_1,in Kidney

目的:探讨酒精引起的大鼠肾损伤模型的病理学改变及肾组织中转化生长因子β_1(TGF-β_1)的表达。方法:以酒精灌胃法制备大鼠酒精性肾损伤模型,以光镜和电镜观察肾组织病理改变,免疫组化染色观察肾组织中TGF-β_1的表达。结果:光镜下HE染色可见模型组肾组织在相应阶段出现肾小管间质内炎性细胞浸润,上皮细胞空泡变性肿胀,管腔闭塞等;电镜下模型组肾组织在相应阶段出现间质内的纤维成分增多;免疫组化染色模型组肾小管上皮细胞内TGF-β_1呈阳性表达,随酒精负荷时间的增加阳性表达增加(P<0.01)。结论:大鼠酒精性肾损伤以肾小管—间质的炎性改变及形成肾间质纤维化为主要病理特征:酒精性肾损伤模型机制可能与肾组织中TGF-β_1的阳性表达明显增加有关。

Objective：To investigate the pathological changes and the expression of TGF-β1, in renal impairment model induced by alcohol in rats. Methods： Experimental model of alcoholic kidney injury was established in rats with oral administration of alcohol. Histopathological changes in kidney were observed by light microscopy and electron microscopy. Immunohistochemistry was used to detect the expression of TGF-β1. Results： Inflammatory cells tissure infiltrated into tubulointerstitial： Epithelial ceils was vacuolar degeneration, tubules was obliterated, and fiber tissue increased in the kidney. The expression of TGF-β1, was positive in the tubular cells and increased with the alcoholic loading time extending. Conclusion： The alcoholic renal injury in rats was characterized by inflammation of tubulointerstitial tissue and fibrosis of interstitial tissue,which may be related to the increasing expression of TGF-β1.