卵巢癌中myc基因家族扩增的初步分析

Studies of the amplification rate of myc gene family in ovarian carcinoma tissue

本研究采用聚合酶链反应（PCR）结合中性聚丙烯酰胺凝胶电泳和激光扫描技术，对38例卵巢癌、20例癌旁组织、4例卵巢良性上皮性肿瘤及2例正常卵巢组织进行myc基因家族检测，发现在卵巢癌组织中C－myc、N－myc、L－myc扩增率分别为47％、42％、42％。癌旁组织扩增率分别为40％、20％、40％。在淋巴结转移的10例卵巢癌标本中，C－myc与N－myc全部扩增，L－myc扩增只有2例，良性肿瘤及正常组织均未有扩增。结果还显示：L－myc和N－myc同时扩增占16％（6／38），N－myc和C－myc同时扩增占26％（10／38），而C－myc和L－myc同时扩增占16％（6／38），没有一例三种基因同时扩增。大多数扩增与临床分期、病理分化程度、淋巴结转移密切相关。

To study the amplification rate of myc gene family in ovarian carcinoma tissues. Methods: In this study, PCR combined with polyacrylamide gel electrophoresis and laser scanning techniques was employed to test myc gene family in 38 ovarian carcinom, 20 paracarcinoma, 4 ovarian benign epithelial tumor, 2 normal ovarian tissues. Results:The amplification rate of C-myc, N-myc, L-myc in ovarian carcinoma was 47%, 42%, 42% respectively, in paracarcinoma tissue was 40%,20%, 40% respectively. C-myc and N-myc were amplified in all emtastasized lymph node of 10 ovarian carcinoma, L-myc was in only 2. No amplification of myc gene family was found in benign tumor and normal tissue. The results also indicated that the simultaneous amplification rate of L-myc and N-myc, N-myc and C-myc, C-myc and L-myc was 16% (6/38), 26% (10/38), 16%(6/38)respectively. Conclusions: The degree of myc gene family amplification was close correlacted with clinical stage, pathological differentiation and lymph node metastasis.