2005-2006南京市食品致病菌污染分析

Analysis on Contamination Status of Food-borne Pathogen in Nanjing From 2005 to 2006

目的了解南京市食源性致病菌在生熟食品中污染状况,提高对食源性疾病预警和控制能力。方法2005、2006年采集6大类食品,共计290份样品,按照GB/T4789-2003食品微生物检验标准[1]进行沙门菌、金黄色葡萄球菌、副溶血性弧菌、单核细胞增生李斯特菌、大肠埃希菌O157∶H7和空肠弯曲菌6种致病菌检测。菌株鉴定应用ATB细菌鉴定仪,肠毒素检测采用miniVIDAS测试系统。结果2005年150份食品中沙门菌检出率为31%,单核细胞增生性李斯特菌检出率0.7%,O157∶H7大肠埃希菌检出率0.7%;2006年140份食品中沙门菌检出率为4.3%,单核细胞增生性李斯特菌检出率为18%,副溶血性弧菌检出率为25%,金黄色葡萄球菌检出率为20%,O157∶H7大肠埃希菌和空肠弯曲菌未检出。结论2005年食品中沙门菌污染较为严重,2006年单核细胞增生性李斯特菌、金黄色葡萄球菌、副溶血性弧菌污染较为严重,这4种致病菌可能是近年食物中毒发生的潜在危险因素,须引起足够重视。

Objective To understand contamination status of food-borne pathogen in Nanjing and so as to improve alarm and control capability on food-borne diseases. Methods Six kinds of food, totaling 290 samples were collected. These samples were examined for pathogen of Salmonella, Staphylococcus, Vibrio parahaemolyticus, Listeria monocytogenes （LM）, Escherichia coli O157： H7 and Campylobacterjejuni according to criteria of GB/T4789-2003 from 2005 to 2006. ATB was used to identify strains, mini VIDAS was used for testify the enterotoxin. Results In 2005, the overall positive rate of Salmonella was 31%, LM was 0. 7%, O157：H7 was 0.7% in 150 samples, and Campylobacter jejuni hadn＇t been found. In 2006, overall positive rate of Salmonella was 4. 3%, LM was 18%, Vibrio parahaemolyticus was 25%, Staphylococcus was 20%, O157：H7 and Campylobacterjejuni hadn＇t been found in about 140 samples. Conclusion Salmonella contamination was serious in 2005 and contamination of Saphylococcus, Vibrio parahaemolyticus and LM was serious in 2006. They were potential risk factors of food poisoning in recent years, and we should pay attention to them.