尿汞2种测定法评价及成都市尿汞本底值调查

Evaluation on 2 Methods for Examining Mercury in Urine and Background Survey in Chengdu

目的为"修订职业性汞中毒诊断标准实施计划"提供实验室资料。方法对尿汞酸性、碱性冷原子吸引测定2法进行方法学评价,采用《中华人民共和国卫生行业标准》推荐的碱性氯化亚锡还原法和酸性氯化亚锡还原法作方法学评价实验,选择无汞作业史的健康人群做尿汞本底值调查。结果碱性法线性范围0～50μg/L,回收率100.68%,相对标准偏差(RSD)3.39%;酸性法线性范围0～120μg/L,回收率98.84%,相对标准偏差(RSD)3.43%;方法比较实验回归方程:y=0.782+0.346x,r=0.9126。结论2法各项性能指标均可接受,相关性较好,而碱性法操作简便、省时,更适用于临床。成都市尿汞本底值(95%上限)5.61μg/L。

Objective To evaluate 2 methods for examining the urine mercury, and to investigate the urine mercury level of healthy people in Chengdu, so as to provide laboratory data for ＂the Plan to implement the Revised Diagnostic Criterion of Occupational Hydrargyrism. ＂ Methods The alkaline stannous chloride reduction and acidic starmous chloride reduction methods, recommended by the ＂Professional Health Criterion of the People Republic of China＂ were adopted for examining the mearcury in urine Besides, the urine mercury levels of 116 healthy people who have no occupational mercury exposure history were investigated. Results By the alkaline stannous chloride reduction method, the linear range is 0 to 50 μg/L, recycle rate is 100. 68% and relative standard deviation（RSD） is 3. 39% ; by acidic stannous chloride reduction method, the linear range is 0 to 120μg/L, recycle rate is 98. 84% and relative standard deviation（RSD） is 3. 43%. Comparative methodological la- boratory regression equation is y =0. 782 ＋0. 346x, r =0. 912 6. Conclusion Through statistical analysis, indices in above two methods are found to be acceptable, and well related. The tests in both methods have no significant differences, but it is much easier to perform the alkaline method, which is timesaving and more suitable in the clinic. The urine mercury（with 95% upper limit）of Chengdu healthy people is 5. 61 μg/L.