摘要
对垂柳愈伤组织和植株再生体系进行了研究,结果显示茎段比较适合诱导愈伤组织,质量浓度为1.0mg/L和2.0mg/L的6-BA均能诱导垂柳茎段愈伤组织;MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+KT 0.5 mg/L的激素配比最为适合胚状体和芽的分化;MS+6-BA 1.0 mg/L+Ac 0.3%培养基能促使垂柳根的形成,且能促使芽生长,最终形成幼苗。
In this paper the tissue culture and plant regeneration of Salix babylonica was researched. The results show that the stem is optimum to induce callus; The 6-BA concentration of 1.0 mg/L and 2.0 mg/L can improve callus of Salix babylonicm MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L＋KT 0.5 mg/L is the optimum medium for embryoid and bud induction; MS＋6-BA 1.0 mg/L＋Ac 0.3 % can not only grow root, but also improve bud, and in final regenerated plant.
出处
《河南科学》
2008年第1期42-44,共3页
Henan Science
基金
郑州市重点科技攻关项目(051SGDS17017)
关键词
垂柳
组织培养
植株再生
Salix babylonica
tissue culture
plant regeneration
