没药倍半萜抑制前列腺癌细胞的增殖活性初探

Effect of sesquiterpenoids on proliferation activity of prostate cancer cells

目的初步探讨没药两个倍半萜单体化合物抑制前列腺癌细胞增殖的作用机制。方法应用倍半萜单体化合物处理人前列腺癌细胞株LNCaP后,MTT检测化合物对细胞增殖的影响;流式细胞术进一步分析细胞周期时相的变化;反转录PCR(RT-PCR)检测细胞周期相关蛋白p21WAF/CIP1(p21)和cyclinD的表达水平。结果两个没药倍半萜单体化合物对前列腺癌细胞均有显著的抑制活性(P<0.001),使细胞停滞于G0/G1期;并且能在mRNA水平诱导p21的表达,同时降低cyclinD的表达。结论没药倍半萜化合物抑制前列腺癌细胞增殖,可能是通过上调p21的表达、下调cyclinD蛋白的表达来实现的。

Objective To investigate the effects of sesquiterpenoids on the proliferation of prostate cancer cells. Methods The growth of prostate cancer cells treated with sesquiterpenoids from myrrh was determined by MTT. The cell cycle was analyzed by flow cytometry. Also the expressions of p21^WAR/CIPI （p21） and cyclinD at mRNA levels were determined by reverse transcription PCR （RT-PCR）. Results The two sesquiterpenoids significantly inhibited the proliferation of prostate cancer cells and caused a Go/G1 arrest, also they induced the expression of p21 and decreased the expression of cyclinD. Conclusion Over-expression of p21 and decrease of cyclinD might contribute to the two sesquiterpenoid-mediated inhibitory effects on prostate cancer cell growth.