血塞通对兔下肢缺血再灌注自由基信号的影响

Effect of Xuesaitong on lower limb of rabbit ischemia-reperfusion free radical

目的:探讨血塞通对下肢缺血再灌注损伤的保护作用。方法:采用电子自旋共振技术和电子自旋共振捕集技术(N-tert-butyl-2-phenylnitrone,PBN)观察兔下肢缺血再灌注时出现PBN-自旋加合物信号。结果:该信号强度与缺血时间有关,缺血4h再灌注时的信号明显强于缺血2h再灌注;血塞通对该自旋加合物信号有明显猝熄作用。结论:血塞通对下肢缺血再灌注损伤具有一定的保护作用。

Objective： To investigate the protective effect of Xuesaitong （total saponins of sanchi） on lower extremity ischemical reperfusion injury. Methods： To observe PBN -spin bulky adducts signal of lower extremity ischemical reperfusion of rabbit by using electron spin resoance technology and electron spin resonance promoter N-tert-butyl-2-phenylnitrone （PBN）. Results： The signal intensity is correlated with time, compared with ischemia for 2 hours, the signa/intensity of ischemia for 4 hours increased apparently. Xuesaitong would quench PBN -spin bulky adducts signal significantly. Conclusion： Xuesaitong have protective effect on lower extremity ischemia-reperfusion injury.