摘要
利用杆状病毒表达系统在家蚕BombyxmoriL.细胞BmN中表达家蚕30K蛋白,以亲本病毒BmPAK6为对照,将重组病毒Bm/r-30K分别感染BmN细胞及家蚕幼虫,观察其感染后不同时间的凋亡及存活率。与对照相比,重组病毒感染后的BmN细胞的存活率明显高于对照组,并且感染的家蚕幼虫存活时间也较长,表明体内过量表达家蚕30K蛋白有助于延长其细胞及幼虫的存活。
The 30K protein was successfully expressed in the Bombyx mori L. （Bm） cells by baculovirus system. B. mori cells and larvae were infected with the recombinant baculovirus（ Bndr-30K）, respectively. The viability of cells and larvae were assayed at various times post-infection and BmPAK6 was used as a control. The viability of BmN cells infected with baculovirus（Bndr-30K） was higher than that of the cells infected with BmPAK6 virus, the viability and surviving time of B. mori larvae were increased as well. The results indicate that over-expression of the B. mori 30K gene contributes to increase the＇surviving time of BmN cells and larvae.
出处
《昆虫知识》
CSCD
北大核心
2007年第6期826-829,共4页
Entomological Knowledge
基金
973课题:家蚕生物工程及基因组应用的拓展研究(2005CB121005)
江苏省教育厅高校科学研究项目:家蚕细胞凋亡的关键基因克隆和鉴定(02KJD18003)
江苏大学自然科学创新预研基金项目(04CX08)
关键词
家蚕
30K蛋白
杆状病毒
表达
生长
Bombyx mori, 30K protein, baculovirus, expression, longevity
