萜类化合物对小菜蛾幼虫的拒食活性

Antifeedant activity of terpene compounds against larvae of the diamondback moth,Plutella xylostella

采用叶碟浸液法测试从松节油合成的26个不同结构萜类化合物样品对小菜蛾Plutella xylostella(L.)4龄幼虫的拒食活性。结果显示,编号为2,4,8,13,27,31的6个化合物,即诺卜醇、诺卜丙基醚、内型异莰烷基甲醇丙酸酯、4-(1-甲基乙烯基)-1-环己烯-1-乙醇丙酸酯、羟基香茅醛丙酸酯、羟基香茅醛1,2-丙二醇缩醛具有较高的拒食活性。处理有效成分为0.01g/mL时,这6个化合物24h拒食率为70%～100%。其中2,4,8号3个化合物拒食活性最高,处理有效成分为0.01g/mL和0.001g/mL时,24h拒食率分别为98.33%,99.80%,100.00%和85.60%,83.90%,66.97%;并且持效性较好,72h拒食率分别为92.67%,89.97%,98.73%和63.20%,63.30%,45.93%。

The antifeedant activities of twenty six terpene compounds synthesized from turpentine were tested with 4th instar larvae of diamond back moth, Plutella xylostella （ L. ）, using dipped cabbage discs. Six of these compounds, numbered 2, 4, 8, 13, 27 and 31, namely nopol, nopyl propyl ether, endo-isocamphanyl methanol propionate, 4- （1-methyl ethylene）-1-cyclohexenyl-1-ethol propionate, hydroxycitronellal propionate and hydroxycitronellal 1,2- propanediol acetal, showed rather high antifeedant activity, and compound 2, 4 and 8 displayed the highest. Compounds 2, 4 and 8 held 98,33%, 99.80% and 100.00% antifeedant rate respectively 24 hours later, and 92.67%, 89.97% and 98.73% 72 hours later when the leaf discs were treated at the dosage of 0.01 g/mL. While the dosage was 0.001 g/mL, the antffeedant rates were 85.60%, 83.90% and 66.97% 24 hours later, and 63.20% , 63.30% and 45.93% 72 hours later respectively. The results also showed that the antifeedant activity went high with the dosage increase, down with the time going.