己烯雌酚对成年雄性金色中仓鼠的生殖毒性与氧化损伤的关系

Relationship between reproductive toxicity and oxidative damage induced by diethylstilbestrol in adult male golden hamsters

为研究环境雌激素己烯雌酚(DES)的生殖毒性与活性氧(ROS)的关系,连续7天给成年金色中仓鼠皮下注射0、0.01、0.1、1mg/kgBWDES,称量睾丸重量、计算睾丸相对重量,光镜观察睾丸组织结构的变化,分光光度法检测睾丸组织和血浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)和丙二醛(MDA)的含量。结果表明:1mg/kgBWDES导致睾丸萎缩、重量下降,曲细精管中生精细胞排列紊乱,管腔内几乎没有成熟精子;随着DES剂量的增加,睾丸组织中SOD、GSH-Px和T-AOC含量显著下降,MDA显著上升。提示DES的生殖毒性与ROS密切相关,DES通过降低抗氧化酶水平,增加ROS含量,干扰生精细胞正常功能,导致细胞死亡,表明氧化损伤可能是环境雌激素生殖毒性的作用机理之一。

To explore the relationship between reproductive toxicity and oxidative damage induced by diethylstilbestrol （DES）, adult male golden hamsters （ Mesocricetus aaratus ） were treated with different dosages of DES （0, 0.01, O. 1, 1 mg/kg body weight） by subcutaneous injection for 7 consecutive days. Absolute and relative testicular weights were measured. Morphological changes in testes and spermatogenetic cells were observed in the light microscope. The contents of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, total antioxide capacity （T-AOC） and malondialdehyde （MDA） in testicular tissue and plasma were detected by spectrophotography. Results showed that testicular weight decreased significantly with increasing dosages of DES. Germ cells were scattered irregularly in seminiferons tubules and few mature sperms were observed in the lumens. As well as morphological changes, the antioxidant enzymes including SOD, GSH-Px and T-AOC in testicular tissue all decreased which led to the increase in MDA. It is concluded that there is a close relationship between reproductive toxicity of DES with reactive oxygen species （ROS）. DES impairs the normal function of spermatogenic cells through decreasing antioxidase avtivity, resulting in the increases of ROS. These results indicate that oxidative damage may be one of the mechanisms of reproductive toxicity induced by environmental estrogens [Acta Zoologica Sinica 53 （6）： 1076- 1082, 2007].