健康足月分娩新生儿泌尿生殖道解脲脲原体的流行病学研究

Molecular epidemiological investigation of Ureaplasma urealyticum in urogenital tract of full term infants

目的:确定解脲脲原体(UU)在健康足月分娩新生儿泌尿生殖道中的检出率与分群分型。方法:UU液体培养法初筛健康足月分娩248例新生儿外阴分泌物,阳性标本用PCR法及酶切法进行分群分型。结果:248例标本中,UU阳性率为10.48%,经阴道分娩组与剖宫产组阳性率差异有统计学意义(P<0.01);男婴与女婴阳性率差异有统计学意义(P<0.01)。26例阳性标本中,生物一群18例(69.23%),生物二群6例(23.08%),混合群2例(7.69%)。生物一群中1型4例,6型10例,3/14型2例,混合型2例。结论:本研究中健康新生儿泌尿生殖道中UU以生物一群为主,主要为1型和6型。

Objective： To determine the detection rate and serotyping of Ureaplasma urealyticum （UU） in urogenital tract of full term infants. Methods： Swab samples taken from cunnns in 248 full term infants were cultured by UU selective hquid medium. The serotyping was determined by PCR method and restriction enzyme excision. Results： The detection rate of UU in 248 healthy neonates was 10.48%. The detection rates of UU between infants dehvered via vaginal and delivered by cesarean, and between male and female had significant differences （ P 〈 0.01 and P 〈 0.01, respectively）. In 26 UU positive specimens, 18 were parvum biovar （69.23%）, 6 urealyticum biovar （23.08%） and 2 mixed biovars （7.69%）. In parvum biovar, there were serotype 1（4 cases）, serotype 6（ 10 cases） and serotype 3/14（2 cases）. Conclusion： The main boivar of the isolates is parvum biovar and 1, 6 serotypes in the current study.