白介素10对实验性急性坏死性胰腺炎胰腺缺血的影响

Effect of Interleukin 10 on Pancreatic Ischemia of Experimental Acute Necrotizing Pancreatitis

目的通过建立大鼠急性胰腺炎(acute necrotizing pancreatitis,ANP)模型,探讨白介素10(IL-10)对急性坏死性胰腺炎胰腺缺血的影响,进一步探讨ANP的发病机制,观察IL-10对ANP的治疗作用。方法将92只SD大鼠随机分为正常对照组(C组)、ANP模型组(A组)、IL-10干预组(I组)。C组接受生理盐水对照;A组用大剂量腹腔注射左旋精氨酸(L-Arginine)的方法诱导ANP模型;Ⅰ组在诱导ANP模型后应用IL-10后干预。分别观察各组光镜下病理评分、血清淀粉酶、白介素1β(IL-1β)、血栓素A2(TXA2)、前列环素(PGI2)的稳定代谢产物浓度TXB2和6-keto-PGF1α浓度变化。结果A组的病理评分、血清淀粉酶、IL-1β、TXB2、6-keto-PGF1α浓度在4、12、24、48时点都明显升高,与C组对比皆有显著差异(P<0.05);应用IL-10干预后的Ⅰ级组大鼠胰腺损伤较轻。结论胰腺缺血是急性坏死胰腺炎的损害因素之一,IL-10可以通过细胞因子网络改善胰腺缺血,对ANP有一定的治疗作用。

Objective To evaluate the effects of interleukin 10 on pancreatic ischemia of experimental acute necrotizing pancreatitis（ANP）. Methods 92 rats were randomly divided into control group（C, n =24） ,acute pancreatitis group（A, n =36） and IL-10 group（ I , n =32）. The acute pancreatitis model in rats were induced by intraperitoneal injection of 6% L-arginine. Group I was treated with 10 000 units of intraperitioneal recombinent human IL-10 at 2nd,5th and 8th hour after the L-arginine injection. Rats were killed at 4th, 12th,24th and 36th hour after the last L-arginine injection. Histologic score, the levels of amylase, interleukin-113 （IL-1β） , thromboxane BE （TXB2 ） ,6-keto-PGF1α of serum was observed. Results Compare with group C, the level of histologic score, serum amylase, IL-1β,6-keto-PGF1α compare with group C, the level of histo- logic score, serum amylase, IL-1β,6-keto-PGF1α and TXB2 of group A and was significantly（ P 〈 0. 05 ） higher at different time. After treating with IL-10, the histologic score and serum levels of amylase, IL-1β, TXB2 ,were significantly decreased. Conversely, the level of serum 6-keto-PGFlα was increased. The TXB2/6-keto-PGF1α ratio was diminished too. Pancreatitis damage in rats of group I was slighter. Conclusion Microeirculatory disturbance is an important impairment factor in acute pancreatits IL-10 can improve the pancreatic ischemia of pancreas in ANP rats by keeping the balance of TXBE/6-keto-PGF1α ratio and may be an effective drug for acute pancreatitis treatment.