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TIMP-1降低无血清培养所致的乳腺癌细胞MCF-7凋亡的研究

Effect of TIMP-1 on decreasing apoptosis induced by serum-free in breast carcinoma cell line MCF-7
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摘要 目的:基质金属蛋白酶组织抑制剂-1(tissue inhibitor of metalloproteinase-1,TIMP-1)是与乳腺癌预后不良显著相关的分子。本研究旨在探讨TIMP-1的作用机制是否包括其降低无血清所导致的细胞凋亡。方法:对TIMP-1表达质粒进行酶切及序列测定;用脂质体转染的方法将含TIMP-1的质粒及对照质粒分别转染到MCF-7细胞中,Western blot检测TIMP-1蛋白的表达;在无血清培养条件下,流式细胞仪检测对照质粒转染克隆与TIMP-1稳定表达克隆凋亡的变化。结果:与转染对照质粒的克隆相比,TIMP-1转染克隆中TIMP-1蛋白的表达显著上调。无血清培养条件下,对照克隆细胞相对凋亡率为(7.18±1.12)%,TIMP-1表达克隆T10和T13相对细胞凋亡率分别为(2.63±0.39)%和(1.07±0.9)%(P<0.05)。结论:TIMP-1过表达降低无血清所导致的细胞凋亡。 Objective：To investigate whether TIMP-1 decreased apoptosis induced by serum-free. Methods：TIMP-1-expression plasmid was identified by enzyme digest and sequencing; The plasmid containing TIMP-1 cDNA and control plasmid were transfected respectively into MCF-7 cells by lipofectamine. Then the TIMP-1 protein was identified by western blot; Cell apoptosis for control clones and TIMP-1 over-expression clones was analyzed by cytometry in the condition of serum-free. Results：The TIMP-1 protein expression level of TIMP-1-transfected clones was significantly higher than that of control clones. The relative apoptosis rate was （7.18±1.12）% for control clones and （2.63±0.39）%, （1.07±0.9）% for T10 and T13 clones, respectively. Conclusion：TIMP-1 decreased apoptosis induced by serum-free.
作者 周平 高萍 王婷
机构地区 江苏省肿瘤医院标本库
出处 《临床肿瘤学杂志》 CAS 2007年第12期885-887,892,共4页 Chinese Clinical Oncology
基金 江苏省高校自然科学基础研究项目(06KJB310068) 江苏省科技发展计划项目(BS2006006)
关键词 TIMP-1 无血清培养 MCF-7 细胞凋亡 TIMP-1 Serum-free culture MCF-7 Apoptosis
分类号 R737.9 [医药卫生—肿瘤]
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参考文献8

  • 1Wurtz SO, Schrohl AS, Sorensen NM, et al. Tissue inhibitor of metalloproteinases-1 in breast cancer[ J]. Endocr Relat Cancer,2005, 12(2):215 -227.
  • 2Lambert E, Boudot C, Kadri Z, et al. Tissue inhibitor of metalloproteinases-1 signalling pathway leading to erythroid cell survival[J]. Biochem J, 2003, 372(3) :767 -774.
  • 3Lee SJ, Yoo HJ, Bae YS, et al. TIMP-1 inhibits apoptosis in breast carcinoma cells via a pathway involving pertussis toxin-sensitive G protein and c-Src [ J ]. Biochem Biophys Res Commun, 2003, 312(4) :1196 - 1201.
  • 4Liu XW, Bemardo MM, Fridman R, et al. Tissue inhibitor of metalloproteinase-1 protects human breast epithelial cells against intrinsic apoptotie cell death via the focal adhesion kinase/phosphatidylinositol 3-kinase and MAPK signaling pathway[ J]. J Biol Chem, 2003, 278(41 ) :40364 -40372.
  • 5Liu XW, Taube ME, Jung KK, et al. Tissue inhibitor of metaltoproteinase-1 protects human breast epithelial cells from extrinsic cell death: a potential oncogenic activity of tissue inhibitor of metalloproteinase-1 [ J]. Cancer Res, 2005, 65 ( 3 ) :898 - 906.
  • 6Schrohl AS, Holten-Andersen MN, Peters HA, et al. Tumor tissue levels of tissue inhibitor of metalloproteinase-1 as a prognostic marker in primary breast cancer[J]. Clin Cancer Res, 2004, 10 (7) :2289 -2298.
  • 7Schrohl AS, Meijer-van Gelder ME, Holten-Andersen MN, et al. Primary tumor levels of tissue inhibitor of metalloproteinases-1 are predictive of resistance to chemotherapy in patients with metastatic breast cancer[ J ]. Clin Cancer Res, 2006, 12 (23) : 7054 - 7058.
  • 8孙静霞,王婷.紫杉醇调节TIMP-1启动子活性及TIMP-1降低紫杉醇对乳腺癌细胞增殖抑制作用的研究[J].医学分子生物学杂志,2007,4(6):484-488. 被引量：1

二级参考文献14

  • 1[1]WURTZ S O,SCHROHL A S,SORENSEN N M,et al.Tissue inhibitor of metalloproteinases-1 in breast cancer[J].Endocr Relat Cancer,2005,12:215-227.
  • 2[2]SCHROHL A S,CHRISTENSEN I J,PEDERSEN A N,et al.Tumor tissue concentrations of the proteinase inhibitors tissue inhibitor of metalloproteinases-1 (TIMP-1) and plasminogen activator inhibitor type 1 (PAI-1) are complementary in determining prognosis in primary breast cancer[J].Mol Cell Proteomics,2003,2:164-172.
  • 3[3]HANKE B,WEIN A,MARTUS P,et al.Serum markers of matrix turnover as predictors for the evolution of colorectal cancer metastasis under chemotherapy[J].Br J Cancer,2003,88:1248-1250.
  • 4[4]CLARK I M,RPWAN A D,EDWARDS D R,et al.Transcriptional activity of the human tissue inhibitor of metalloproteinases 1 (TIMP-1) gene in fibroblasts involves elements in the promoter,exon 1 and intron 1[J].Biochem J,1997,324:611-617.
  • 5[5]EDWARDS D R,ROCHELEAU H,SHARMA R R,et al.Involvement of AP1 and PEA3 binding sites in the regulation of murine tissue inhibitor of metalloproteinases-1 (TIMP-1) transcription[J].Biochim Biphyso Acta,1992,1171:41-55.
  • 6[6]HENRIET P,BLAVIER L,DECLERCK Y A.Tissue inhibitors of metalloproteinases (TIMP) in invasion and proliferation[J].Apmis,1999,107:111-119.
  • 7[7]BLAVIER L,HENRIET P,IMREN S,et al.Tissue inhibitors of matrix metalloproteinases in cancer[J].Ann N Y Acad Sci,1999,878:108-119.
  • 8[8]LAMBERT E,BOUDOT C,KADRI Z,et al.Tissue inhibitor of metalloproteinases-1 signalling pathway leading to erythroid cell survival[J].Biochem J,2003,372:767-774.
  • 9[9]HAYAKAWA T,YAMASHITA K,TTANZAWA K,et al.Growth-promoting activity of tissue inhibitor of metalloproteinases-1 (TIMP-1) for a wide range of cells.A possible new growth factor in serum[J].FEBS Lett,1992,298:29-32.
  • 10[10]LI G,FRIDMAN R,KIM H R,et al.Tissue inhibitor of metalloproteinase-1 inhibits apoptosis of human breast epithelial cells[J].Cancer Res,1999,59:6267-6275.
临床肿瘤学杂志
2007年 第12期

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