魟鱼软骨多糖抗血管形成的实验研究

Anti-angiogenic Activity of Ray Cartilage Glycosaminoglycans:An Empirical Study

目的:探讨魟鱼软骨多糖(RCG)对血管形成的作用。方法:原代培养人脐静脉内皮细胞,采用MTT法检测不同浓度RCG对人脐静脉内皮细胞增殖的影响;缝线诱导大鼠角膜新生血管,通过计算新生血管面积评价不同浓度RCG对大鼠角膜新生血管的作用;建立小鼠Lewis肺癌模型,观察不同浓度RCG作用后肿瘤生长情况,计算原发瘤抑制率,免疫组织化学染色计数肿瘤组织微血管密度(MVD)。结果:RCG可明显抑制人脐静脉内皮细胞的体外增殖,IC50为62.93mg.mL-1;与生理盐水组比较,应用不同浓度RCG后新生血管面积明显减少(P<0.01),原发瘤抑制率呈浓度依赖性,MVD值降低(P<0.01)。结论:RCG对实验动物具有良好的抗血管生成活性。

OBJECTIVE： To investigate the anti- angiogenic activity of ray cartilage glycosaminoglycans （RCG） METHODS： Primary culture of human umbilical vein endothelial cells （HUVEC） was performed, and MTT was adopted to evaluate the effects of RCG on the proliferation of HUVEC; Corneal neovascularization （CNV） was induced by sutures on Wistar rats, and CNV area was calculated to evaluate the effects of different concentration of RCG on CNV in Wistar rats. The model of mice with Lewis lung carcinoma was made to observe the effects of different concentration of RCG on tumor growth, with inhibition ratio of primary tumor measured and microvessel density （MVD） quantitated by immunohistochemical staining. RESULTS： RCG obviously inhibited the proliferation of HUVEC in vitro, with IC50 value at 62.93 mg · mL^ -1. As compared with normal saline group, RCG groups showed smaller areas of new blood vessels （P 〈 0.01）, and the inhibition ratio of primary tumor assumed a concentration-dependant manner and in which microvessel density was decreased （P〈0.01） . CONCLUSION： RCG showed a satisfactory anti- angiogenic activity in the experimental animals.