正交试验优选幽门螺旋杆菌培养条件

Optimizing the Best Medium of HP by Orthogonal Test Design

目的:优化幽门螺旋杆菌培养处方。方法:采用正交试验设计,优选幽门螺旋杆菌固体培养处方,并研究其菌种保存条件。结果与结论:不同培养处方条件下幽门螺旋杆菌生长情况存在显著差异;幽门螺旋杆菌最佳固体培养条件为92%哥伦比亚琼脂或脑心浸液琼脂加5.0%牛血清、再加2.5%混合抗生素,pH值为7.5。菌株采用脑心浸液加甘油制成的冷冻保存液于—70～—80℃低温条件下保存,可以成功复苏。

OBJECTIVE：To select the best medium prescription of HP. METHOD： To select the best solid medium of HP by orthogonal test design and to study the condition of preserving HP. RESULTS ＆ CONCLUSIONS： There are significant deviations of growing circumstance of HP on the different cultivation medium. The best solid cultivation medium for HP is colombian agar or brain heart infusion agar medium with 2.5%mixed antibiotic added with 5.0%bovine serum and kept the pH of medium at 7.5. HP is preserved under -70 ℃ --80 ℃ , which could be successfully antibiotic.