甲鱼油对胰岛素抵抗细胞模型胰岛素敏感性和葡萄糖代谢的影响

Effects of Turtle Oil on Insulin Sensitivity and Glucose Metabolism in Insulin Resistant Cell Model

采用高浓度胰岛素诱导建立胰岛素抵抗（IR）HepG2细胞模型，评价甲鱼油对IR细胞模型胰岛素敏感性和葡萄糖代谢的影响。利用甲鱼油处理IR细胞模型，通过葡萄糖氧化酶及^3H-D葡萄糖参入实验分别检测其葡萄糖消耗和^3H-D-葡萄糖参入率。结果表明，HepG2细胞模型的葡萄糖参入率在各种浓度的胰岛素刺激下均低于对照细胞（P〈0．05），并且HepG2细胞模型与对照细胞的^3H-D-葡萄糖参入率随着胰岛素浓度的升高而升高，但前者升高的幅度低于后者。MTT（甲基噻唑基四唑）结果显示甲鱼油均具有促进对照细胞和IR细胞模型增殖作用。与对照组细胞相比，采用不同浓度胰岛素诱导的模型细胞经甲鱼油处理后其葡萄糖摄取和消耗显著增加（P〈0．05）。甲鱼油明显增加该IR细胞模型的葡萄糖消耗和^3H-D-葡萄糖参入率，提高IR细胞模型的胰岛素敏感性，改善其糖代谢。

To evaluate the effects of turtle oil on insulin sensitivity and glucose metabolism in an insulin-resistant （IR） cell model which was established by the way of high concentration of insulin induction with HepG2 cell in vitro culture. The IR cells were treated by turtle oil, the glucose consumption and 3 H-D-glucose incorporation rate in IR cells were detected by the way of glucose oxidase and 3 H-D-glucose incorporation assay respectively. The state of cell proliferation was tested by MTT method. The results showed that the incorporation rate of 3 H-D-glucose in IR cells was significantly lower than that in the control cells（P〈0.05）. The 3 H-D-glucose incorporation rate in either IR cells or control cells was increased with the increase of insu- lin concentration. Moreover, the 3 H-D-glucose incorporation rate of IR cells increased slower than that of control cells. The MTT assay showed that turtle oil can promote the proliferation of IR cell and control cell. The glucose uptake and glucose consumption in IR cell which treatedwith turtle oil was significantly increase than that in the control cells（P〈0.05）. Turtle oil can improve the insulin sensitivity and glucose metabolism in the IR cell model.