硫噻蒽的光化学荧光特性

Photochemical Fluorescence Nature of Thiothixene

在弱碱性介质中，硫噻蒽在紫外光照射下能发生光化学反应而形成具有强荧光的产物，λｅｘ为２６７、３０９和３８７ｎｍ，λｃｍ为４４２ｎｍ。在硫噻蒽浓度为０．００１～０．５０ｍｇ／Ｌ范围内，荧光强度与浓度呈良好的线性关系，据此建立了光化学荧光测定硫噻蒽的新方法。方法的检出限为０．２０μｇ／Ｌ，相对标准偏差为１．４％。本法简便、快速、灵敏度高，可望应用于血清中硫噻蒽的测定。

Thiothixene, a nonfluorescent substance, is transformed into a highly fluorescent product (λcx 267, 309 and 387 nm, λcm = 442 nm) under the irradiation of ultraviolet light in weak alkaline medium. Thus, a photochemical fluorimetric method for the determination of thiothixene was developed with a detection limit of 0. 20 μg/L and a relative standard deviation of 1. 4%. The linear relationship between the fluorescence intensity and the concentration of thiothixene is over the range of 0. 001-0. 50 mg/L. This method is simple, rapid and sensitive, and is expected to be applied to the determination of thiothixene in serum.