CRP促进THP-1来源的巨噬细胞血凝素样氧化型低密度脂蛋白受体-1的表达

CRP promotes lectin-like oxidized low density lipoprotein receptor-1 expression on THP-1 derived macrophages

目的探讨C反应蛋白(CRP)对单核细胞株(THP-1)来源的巨噬细胞血凝素样氧化型低密度脂蛋白受体-1(LOX-1)蛋白和mRNA的表达及相关信号转导通路的影响。方法用佛波醇脂诱导THP-1分化为巨噬细胞。用不同浓度CRP在体外干预,分别采用RT-PCR和细胞酶联免疫测定干预后巨噬细胞LOX-1抗原蛋白和mRNA的表达。同时观察当NF-κB、AP-1和MARK信号通路抑制剂存在时,CRP对LOX-1抗原和mRNA表达的影响。结果CRP促进巨噬细胞LOX-1蛋白和mRNA表达增加。NF-κB的抑制剂BAY11-7085能抑制CRP对LOX-1蛋白和mRNA表达的诱导作用。结论CRP能在转录及转录后水平诱导THP-1来源的巨噬细胞表达LOX-1,这种调节作用可能是通过NF-κB信号传导通路实现的。

Objective To explain the effects of C-reactive protein （CRP） on lectin-like oxidized low density lipoprotein receptor-1 expression on THP-1 derived macrophages and the related signal transduction pathways. Methods THP-1 cells were differentiated into macrophages with the stimulation of PMA. THP-1 derived macrophages were incubated with CRP and co-incubated with inhibitors of NF-κB,AP-1 and MARK signal transduction pathways. The expression of LOX-1 antigen and mRNA was analyzed by ELISA and RT-PCR. Results CRP stimulated the expression of LOX-1 antigen and mRNA on macrophages in a dose-dependent manner. NF-KB inhibitor BAY11-7085 suppressed the inducible effects of CRP on LOX-1 expression. Conclusion CRP increased LOX-1 expression on THP-1 derived macrophages at transcription and post-transcription levels. The NF-κB signal transduction pathway may be involved in such process.