自体外周血干细胞移植促进患者下肢动脉缺血性溃疡愈合

Transplantation of autologous peripheral blood stem cells accelerates ulcer cicatrisation in ischemic disease of lower limb arterial

目的探讨应用自体外周血干细胞移植技术治疗患者下肢动脉缺血性溃疡和静息痛可行性及存在的问题。方法自2004年3月—2007年2月，16例下肢动脉缺血性溃疡及静息痛患者通过采用重组人粒细胞集落刺激因子（G-CSF）动员骨髓干细胞增殖并释放入血，在第5—6天应用血细胞分离机进行外周血干细胞的采集，并将采集到的浓集干细胞液作患肢小腿肌肉注射。结果16例患者经干细胞移植治疗后间歇性跛行时间及距离均延长，其中，12例患者静息痛均缓解；11例溃疡愈合。病人均随访3—24个月。4例失访。结论应用自体外周血干细胞移植技术治疗下肢动脉缺血性溃疡和静息痛是一种可行且有效的方法。

Objective To evaluate the clinical efficacy of transplantation of autologous peripheral blood stem cells （PBSC） for the treatment of toe or heel ulcer and of rest pain in patients with lower limb arterial ischemic diseases. Methods To mobilize patients＇ own stem cells with G-CSF for 5 days. At the sixth day, PBSC are collected with a blood-cells separator. The PBSC were then intramuscularly injected into ischemic areas of the lower limbs. Results After transplantation, all patients are followed up from 3 to 24 months. The rest pain disappeares in 12 patients,while toe or heel ulcers are cicatrized in 11 cases. However, 4 patients lost in following up. Conclusion Transplantation of autologous peripheral blood stem cells is an effective method for the treatment of arterial ischemic disease.