SCN侵染对不同大豆品种根系氨基酸/低分子肽类分泌物的影响

Amino acids/low molecular weight peptides in root exudation of different soybean cultivars resistance to Heterodera glycines

利用聚酰胺薄膜层析技术研究了不同抗感SCN的大豆品种根系分泌物中氨基酸/低分子肽类物质。结果表明:大豆根分泌物中含有多种氨基酸类物质及谷胱甘肽等低分子肽类物质;大豆胞囊线虫侵染后,大豆根的分泌活动增强,根分泌物中氨基酸/低分子肽类物质增多;苗期大豆根分泌物中氨基酸/低分子肽类物质的种类和含量随苗龄有不同程度的变化,运用SPSS软件对层析图谱进行聚类和相似性分析,结果为出苗5和11 d,各个品种根分泌物层析图谱差异显著,其中Franklin和辽豆10的图谱相似,5和11 d时,r分别为0.6683**和0.7581**(P=0.01),且二者均为苗期线虫侵入型品种。因此,以Franklin或辽豆10根分泌物层析图谱荧光点作参照,聚酰胺薄膜层析法可检测大豆苗期是否抗线虫侵入。

Amino acids/low weight molecular peptides in root exudation from different soybean cultivars were studied by the method of polyamide thin layer chromatography. The results showed that analog of amino acids and glutathion in soybean root exudation could be detected by the method. Soybean root excreted more analog of amino acids/low weight molecular peptides after inoculated with Heterodera glycines. The sort and concentration of analog of amino acids/low weight molecular peptides were changed with the growth of seedling. The correlation of fluorography spots distribution in different patterns of polyamide thin layer chromatography was analyzed by SPSS software. The results suggested there were significant differences in different patterns at 5 and 11 d after seedling, chromatography profiles of root exudation from Liaodou 10 and Franklin were obviously similar, correlation coefficients of 5 and 11 d were 0. 668 3^＊＊ and 0. 758 1^＊＊ respectively （P =0.01 ）, and the two cultivars were infected by abundance nematode during seedling stage. By using the pattern of Franklin or Liaodou 10 as control, invasion-against nematode of the soybean cultivars could be determined by the polyamide thin layer chromatography method at seedling stage.