四君子汤药膳对严重烫伤大鼠免疫功能紊乱的调理作用

Experimental research on the effects of Chinese herb drugs on immune dysfunction in severely scalded rats

目的了解四君子汤药膳对严重烫伤大鼠免疫功能紊乱的调理作用。方法将90只健康Wistar大鼠造成30%TBSAⅢ度烫伤后,按照完全随机法分为药膳喂养组30只、肉汤喂养组30只、常规喂养组30只;另设对照组10只(模拟烫伤)。药膳喂养组大鼠烫伤后按常规复苏及处理创面,分笼饲养,并于伤后2h开始向胃内灌注37℃的四君子汤药膳,2ml/次、2次/d;肉汤喂养组除灌注肉汤外,其余处理同前;常规喂养组伤后常规处理,自由进食、饮水。前3组大鼠于伤后3、7、14d检测T淋巴细胞亚群和天然杀伤(NK)细胞的活性,血浆IgA、IgG、IgM、C3、C4水平,肠道分泌型IgA (sIgA)水平;对照组亦行相应检测。结果前3组大鼠伤后大部分时相点各项指标(除CD8^+T淋巴细胞活性外)均低于对照组(P<0.05或P<0.01)。伤后3～14d,药膳喂养组sIgA水平为(50±8)～(57±11)μg/ml,明显高于其他2个致伤组水平(P<0.05或P<0.01);其余免疫指标组间比较结果与此相似。结论四君子汤药膳可以改善严重烫伤大鼠T淋巴细胞亚群的分布,提高NK细胞活性,促进血浆IgA、IgM、IgG、C3、C4水平的恢复和肠黏膜细胞sIgA的分泌,从而改善机体免疫功能。

Objective To observe the modulation effect of a concoction of Chinese herb drugs on immune dysfunction in severely burned rats. Methods One hundred healthy Wistar rats were randomly divided into A group （ n = 30,with Chinese herb drug feeding after burns） , B group （ n = 30, with bouillon feed- ing after burns） , C group （ n = 30, with ordinary feeding after burns） , and D group （ n = 10, with sham burns as normal controls）. The rats in A ,B and C groups were subjected to 30% TBSA full-thickness burns and received conventional treatment. The rats in A group received 2 ml of Chinese herbal drug at 37 ℃ by gavage two times a day beginning from 2 postburn-hours（PBH）. The rats in B group received 2 ml bouillon by garage instend, and otherwise treatment was the same as A group, while the rats in C group were not fed by gavage. The activity of natural killer cell and T lymphocyte , and the levels of IgA, IgG, IgM, C3 , C4 in A,B,C groups were examined on 3,7,14 PBD, and these indices were also determined in D group. Resalts Compared with D group ,the amount of the CD3 ^＋ , CD4^＋ lymphocyte, the ratio of the CD4 ^＋/CD8^＋ , the level of IgA,IgG,IgM ,C3 , C4 ,the activity of NK cells, and the density of the sIgA in A, B, C groups were obviously decreased , but the amount of the CD8 ^＋ were obviously increased（ P 〈 0.05 or P 〈 0.01 ）. Furthermore, the above indices in A group improved more quickly when compared with B and C groups. Conclusion The concoction of Chinese herb drugs can improve the distribution of T lymphocyte subsets, increase the activity of NK cells ,promote the secretion of sIgA in intestinal mucous membrane and promote recovery of IgM ,IgG,C3,C4 levels, thereby improves the immune function of the body.