MLCT、PCR-SSP检测HLA-B_(27)的比较

Comparsion of micro-lymphocyte cytotoxic test and polymerase chain reaction with sequence specific primers in HLA-B27 antigen detection

目的比较强直性脊柱炎(AS)患者HLA-B27检测方法的特点。方法采用最常见的微量淋巴细胞毒试验(MLCT)、引物特异性聚合酶链反应(PCR-SSP),对30例AS患者及200例无偿献血者的HLA-B27抗原进行检测。结果两种方法之间的HLA-B27检出阳性率差异无统计学意义(P>0.05)。结果两种检测方法均准确可靠,且各具特点。HLA-B27检测对AS的早期诊断及鉴别诊断有重要意义。

Objective To Compare the features of HLA-B27 antigen detection aplied to ankylosing spondylists （AS）. Methods The methods of micro-lynphocyte cytotoxic test （MLCT） and polymerase chain reaction with sequence specific primers （PCR-SSP） were used to test HLA-B27 of 30 patients with AS and 200 volunteer blood donors. Results There was no significant difference in the positive rate of HLA-B27 between two methods （P 〉 0.05 ）. Conclusion The two methods with their features are reliable. HLA-B27 antigen detection is great importance to early diagnosis and differential diagnosis of AS.