川芎嗪对大鼠局灶性脑缺血损伤的神经保护作用

Neuroprotective effect of Ligustrazine on focal cerebral ischemia injury in rats

目的观察川芎嗪对局灶性脑缺血后脑损伤的保护作用。方法采用线栓法制作大鼠左侧大脑中动脉阻塞模型。氯化三苯基四氮唑(TTC)脑片染色测定脑梗死体积,干湿重法测定脑组织含水量,快速Golgi银染方法观察脑缺血周围区神经元的形态改变。结果川芎嗪能明显缩小脑梗死体积、降低脑组织含水量,随着川芎嗪剂量增大,作用更为明显,具有剂量依赖性。脑缺血后14 d Golgi银染显示,模型组在梗死周围区神经元明显减少,变性和正常神经元共存。变性神经元主要表现为突起断裂、增粗,突起有大的串珠,树突棘减少。川芎嗪组较模型组皮质梗死周围神经元变性较少。结论川芎嗪能缩小脑梗死体积、减轻脑水肿、保护缺血周围神经元,证实川芎嗪对脑缺血损伤有保护作用。

Objective To observe the neuroprotective effect of Ligustrazine after focal cerebral ischemia in rats. Methods The model of the middle cerebral artery occlusion （MCAO） was established by placement of an intraluminal filament at the origin of left middle cerebral artery. Volume of cerebral infarction was measured by triphenyltetrazolium chloride （TTC） staining. Cerebral water content was detected ~vith dry-wet weight method. Changes of neurons in peripheral infarction were observed by Golgi silver staining. Results Ligustrazine obviously decreased the cerebral infarction volume and the brain water content, and this effect increased with the increase of Ligustrazine dose. The Golgi silver staining showed that peripheral neurons in the cortex infarction of model group were increased obviously 14 days after ischemia. Degenerative neurons displayed disruption, thickening, and rosary processes, and reduction of dendritic spines. Peripheral neurons in the cortex infarction of Ligustrazine group outnumbered those in the model group, and the neuronal degeneration was less. Conclusion Ligustrazine can reduce the cerebral infarction volume and hydrocephalus, and protect neurons around ischemia area, confirming that Ligustrazine has the protective function on damage caused by cerebral ischemia.