摘要
对拟南芥AtMGT3基因的Mg2+转运功能进行了初步研究.AtMGT3转录本的半定量分析表明,AtMGT3在根、茎、叶、花、角果中均有表达,但在花中表达量最高,根中最少.MM281功能互补和液体生长曲线结果表明:At-MGT3功能互补细菌Mg2+转运突变株,具有Mg2+转运能力;AtMGT3介导Mg2+的低亲和性吸收,是一个低亲和性Mg2+转运蛋白;AtMGT3可能还能转运Fe2+,但转运Fe2+浓度超出了正常的生理浓度.在正常生理条件下,At-MGT3的主要生理功能是作为Mg2+转运蛋白起作用.
The Mg^2＋ transport function of AtMGT3 were primarily studied. The expression pattern indicated that AtMGT3 mRNA existed in all tissues, while mainly in flower and least in root. The results of analyzing of bacterial model and liquid develop curve showed： AtMGT3 functionally complemented a bacterial mutant lacking Mg^2＋ transport capability, mediated low-affinity transport of Mg^2＋ . AtMGT3 may transport other divalent cations like Fe^2＋, but the concentrations required for transport of Fe^2＋ are beyond normal physiological concentrations. Under normal physiological concentrations, the major physiological function of AtMGT3 is Mg^2＋ transport protein.
出处
《生命科学研究》
CAS
CSCD
2007年第4期328-333,共6页
Life Science Research
基金
国家自然科学基金项目资助(30470972)
湖南省杰出青年基金(03JJY1003)
