摘要
采用克隆测序和PCR-RFLP相结合的方法,对民猪的SLA-DRB基因的整个编码区进行了扫描和多态性分析.结果表明该基因的第2外显子是整个基因的高变区,突变率达到5.6%,其中80%为有效突变,但没有发现新的等位基因;PCR-RFLP结果表明外显子1用内切酶AluⅠ酶切可获得3种基因型;外显子2用内切酶TaqⅠ酶切可获得3种基因型;外显子3用内切酶BcnⅠ酶切可获得3种基因型;外显子4用内切酶MboⅠ酶切可获得2种基因型;外显子5用内切酶Hin1Ⅰ酶切可获得3种基因型.Hardy-Weinberg平衡分析表明民猪在AluⅠ和Hin1Ⅰ处于平衡状态(P>0.05),在TaqⅠ、BcnⅠ和MboⅠ处于不平衡状态.
Scan and analysis of polymorphism on whole coding region of SLA-DRB gene by clone and PCRRFLP method in Min pig （Sus scrofa domestica）. The result showed that the exon 2 is the high changed region, mutation rate reached 5.6%, thereinto 80% is effective mutation, but the new allele was not found. 3 genotypes with Alu Ⅰ in exon 1 by PCR-RFLP could be obtained; 3 genotypes with Taq Ⅰ in exon 2; 3 genotypes with Bcn Ⅰ in exon 3; 3 genotypes with Mbo I in exon 4; 3 genotypes with Hinl Ⅰ in exon 5. Min pig is balanced in Alu I and Hinl Ⅰ site （P 〉 0.05）, but unbalanced in Taq Ⅰ ,Bcn Ⅰ and Mbo Ⅰ site by Hardy-Weinberg Equilibrium analysis.
出处
《生命科学研究》
CAS
CSCD
2007年第4期373-376,共4页
Life Science Research
基金
黑龙江省杰出青年基金(JC-05-19)
黑龙江省科技攻关项目(GB05B106)
