同源盒基因HOXC13在牙源性肿瘤中的表达

Expression of HOX C13 in odontogenic Tumors

目的:检测同源盒基因HOXC13 mRNA在牙源性肿瘤中的表达,探讨其发生的意义。方法:采用原位杂交法检测47例成釉细胞瘤(ameloblastoma,AB)(原发29例,复发14例,恶变4例)、3例牙源性钙化囊性瘤(CCOT)、3例成釉细胞纤维瘤(AF)、2例牙源性钙化上皮瘤(CEOT)、10例牙源性角化囊性瘤(KCOT)的HOXC13 mRNA水平,同时选取7例正常口腔黏膜上皮作为对照。采用SPSS10.0软件包对数据进行χ2检验。结果:HOXC13 mRNA在AB中的阳性率为97.9%(46/47),CCOT中为100%(3/3),CEOT中为100%(2/2),KCOT上皮中为70.0%(7/10),正常口腔黏膜细胞中为42.9%(3/7),AB、KCOT、正常黏膜3组间差异显著(Ρ=0.001),但角化及颗粒样变退化细胞却为阴性。3例AF均为阴性。结论:牙源性肿瘤的发生、发展与HOXC13的高表达有关,且受其调控。HOXC13 mRNA在牙源性病损上皮中表达有异质性,该基因可促进上皮增殖,阻抑成釉细胞的终末分化,其丢失可导致上皮细胞角化和退变。

PURPOSE： To study the expression of HOXC13 mRNA in odantogenic tumors. METHODS： HOXC13 mRNA was detected in 47 cases of ameloblastoma （AB）, 3 cases of calcifying cystic odantogenic tumor（CCOT）, 3 cases of ameloblastic fibroma （AF）, 10 cases of keratocystic odontogenic tumor （KCOT） and 2 cases of calcifying epithelial odontogenic tumor （CEOT） by in situ hybridization, and 7 cases of normal oral mucosa were selected as control. SPSS10.0 software package was used for X2 test. RESULTS： HOXC13 mRNA was positively expressed in all odontogenic epithelium except AF. The positive ratios were 97.9% in AB, 100% in CCOT, 100% in CEOT, 70.0% in KCOT epithelium and 42.9% in the normal oral mucosa. There was significant difference among AB, KCOT and normal mucosa （X^2=21.665,P〈0.001）. CONCLUSIONS： The genesis and development of odontogenic tumors are related to the high expression of HOXC13. The expression of HOXC13 mRNA in the odontogenic lesions has heterogeneity.