CyclinD_1和P16在儿童髓母细胞瘤中的表达

THE EXPRESSION OF CYCLIND_1 AND P16 IN MEDULLOBLASTOMAS OF CHILDREN

目的:探讨CyclinD_1、P16在儿童髓母细胞瘤中的表达及临床意义。方法:采用免疫LSABC法,并用图像分析系统测定染色的积分吸光度(IOD)检测84例获随访的儿童髓母细胞瘤中的CyclinD_1,、P16的表达,后者按术后生存周期3、5、10年分为A、B、C三组。结果:①正常小脑组织中CyclinD_1表达较低,A、B、C三组中表达为5.43、3.98和1.02,各组间的比较有统计学差异(P<0.01)②正常小脑组织中P16显现高表达,而A、B、C三组中表达为0.22、1.19和2.85,各组间比较有统计学差异(P<0.01);(3)CyclinD_1与P16的表达具有显著负相关关系(rs=0.7398,P<0.01)。结论:CyclinD_1与P16在儿童髓母细胞瘤预后中,两者表达水平的相关性反映了癌基因与抑癌基因相互协调,相互促进的机制。

Objective：To study the expression and clinical significance of CyclinD1 and P16 in medulloblastoma of children .Methods：A total of 84 children with medulloblastoma and follow-up after operation were divided into group A,B、C by their survival rate of 3,5 and 10 years respectively.The expression of CyclinDland P16 in 84 resected samples was studied with labelled streptayidin biotin method （LSAB）and integral optical density （IOD） value was performed by quantitative image analysis . Results： ①The IOD value of CyclinD1 of normal cerebellar tissues was lower .The IOD valve was 5.43,3.98 and 1.02 respectively in group A ,B,C （P〈 0.01）. ②The IOD value of p16 of normal cerebellar tissues was higher . The IOD value was 0.22,1.19and 2.85 respectively in group A、B and C （P〈0.01）. ③The IOD value of CylinDl and P16 had a negative correlation Conclusion： The results suggest that CyclinDl and P16 play an important role in prognosis processes of medulloblestoma in children .The correlation between CyclinD1 and P16 expression shows the inter-coordination and acceleration mecbailism between oncogene and ani-oncogene .