中药复方“体复康”对运动性疲劳大鼠血乳酸、β-内啡肽、亮氨酸脑啡肽及强啡肽A_(1-13)影响的实验研究

Effects of TCM Preparation Tifukang on Blood Lacate, beta-Endorphin, Leu-Enkephalin and Dynorphin A_(1-13) in the Rat of Fatigue Induced by Exercise

以长时期（共7周）大强度跑台运动（速度由15m／min递增至35m／min，运动时间为20～25min／d）制造大鼠疲劳模型，探索疲劳大鼠外周阿片肽的变化及中药复方的作用机理。结果表明：运动后即刻血乳酸显著高于对照组，血浆β-内啡肽、强啡肽A1-13含量显著下降，而亮氨酸脑啡肽变化不明显；经中药复方治疗后，上述变化均有不同程度的恢复。表明此运动强度下的疲劳大鼠，内源性阿片肽系统受抑制而使外用血中的β-内啡肽、强啡肽A1-13含量下降；中药复方作用后激活了此系统，主要使卜内啡肽水平升高，并对血乳酸有一定的清除作用，从而有利于消除疲劳。

By using the method of treadmill (with the slope at 0 degree) exercise with a long term (totally7 weeks) and high intensity (the speed was from 15 m/min gradually increased to 35 m/min, and the time was 20to 25 min/d), the fatigue model was replicated in order to observe the changes of blood opioid peptides and to findout the therapeutic machanism of Tifukang. The results showed that blood lacate level of model group was significantly higher than that of control group, while the levels of plasma beta-endorphin and dynorphin A_(1-13) were obviously lower, without remarkable change of leucine-enkephalin level. Under the treatment of Tifukang, the abovecriteria were normalized in different degrees. This implies that the endogenous opioid peptides system of the fatiguerat was inhibited by such intensity of exercise, while Tifukang could activate the system and increase plasma betaendorphin level and eliminate blood lactate, which results in recovery from fatigue.