等渗胁迫下PEG和NaCl对野大豆萌发的影响

Effects of PEG and NaCl on the Germination of Glycine soja under Iso-osmotic Stress

[目的]阐明干旱胁迫与盐胁迫的关系,为整体性抗逆研究提供参考。[方法]分别用NaCl、PEG模拟盐胁迫和干旱胁迫,测定等渗透胁迫下野大豆种子的萌发率、发芽势、发芽指数和生长情况。[结果]PEG处理抑制了野大豆的萌发,计数第1天-0.4、-0.8MPaPEG处理的发芽率均明显低于对照。-0.4MPaNaCl促进野大豆的萌发,-1.6MPaNaCl处理发芽率明显降低。低浓度的PEG和NaCl对萌发率影响不大,高浓度的PEG和NaCl降低发芽率,且等渗的PEG对野大豆的发芽率抑制强于NaCl。发芽势、发芽指数的变化与发芽率的变化相似,PEG和NaCl降低种子的萌发势。[结论]野大豆种子对盐胁迫具有更大的萌发耐受能力。在该试验浓度范围内,影响盐生植物种子萌发的因素主要是渗透胁迫。

Objective The aim of the research was to clarify the relationship of drought stress and salt stress and provide basis for the research on stress resistance as a whole Method NaCl and PEG were used to simulate salt stress and drought stress to determine germination rate,the germination potential,the germination index and growth conditions of Glycine soja seeds under iso-osmotic stress.Result PEG treatment inhibited the germination of G.soja and the germination rate of seeds treated with-0.4、-0.8 MPa PEG in the 1st day of counting were obviously lower than that of CK.Treatment with-0.4 MPa NaCl promoted the germination of G.soja and the germination rate in the treatment with-1.6 MPa NaCl was obviously reduced.Low concentration of PEG and NaCl had little effects on the germination rate,high concentration of PEG and NaCl reduced the germination rate and the inhibition effect of iso-osmotic PEG on the germination rate of G.soja was stronger than that of NaCl.The changes of the germination potential and the germination index were similar to that of the germination rate and PEG and NaCl reduced seed germination potential.Conclusion The seeds of G.soja had greater germination tolerance to salt stress.Osmotic stress was main factor that influenced seed germination of halophyte in the concentration rage of this test.