国槐植株再生技术研究

Research on Tissue Culture and Regeneration Technology of Sophora japonica

[目的]筛选国槐组织培养的最佳培养基,建立国槐植株再生体系。[方法]将灭过菌的国槐种子分别接种到4种无菌苗诱导培养基上,待苗长出2～4片真叶时,将叶、下胚轴、子叶分别接种于4种愈伤组织诱导培养基上,然后将子叶产生的愈伤组织分别接种在2种芽分化培养基中,再将继代培养获得的有效苗转入生根培养基,对国槐种子进行无菌苗萌发和植株再生的研究。[结果]培养基的激素配比对种子萌发的影响不大;6-BA能促进愈伤组织的产生;最适宜的胚状体诱导培养基为MS+2.0mg/L6-BA+0.2mg/LNAA+0.5mg/LKT;芽诱导培养基MS+6-1.0mg/LBA+0.1mg/LNAA;生根培养基为1/2MS+0.3mg/LNAA,活性炭不利于国槐的生根培养。[结论]种子的萌发率与国槐的种子质量有关,与培养基的种类关系不大。子叶最适合诱导愈伤组织。

Objective The study aimed to screen out the optimum media for the tissue culture of Sophora japonica and set up its plant regeneration system.Method The sterilized S.japonica seeds were inoculated resp.on 4 kinds of induction media for inducing asepsis seedling,till the seedlings grew out 2～4 leaves,the leaves,hypocotyl and cotyledon were inoculated resp.on 4 kinds of induction media for inducing callus,then the callus grown out from cotyledon were inoculated resp.on 2 kinds of bud differentiation media,finally the effective seedlings obtained through subculture were transplanted into rooting media so as to study the asepsis seedlings germination and plant regeneration of S.japonica seeds.Result The hormone matching of media had little influence on seed germination.6-BA could promote the production of callus.The most suitable induction media was MS＋2.0 mg/L 6-BA ＋0.2 mg/L NAA ＋0.5 mg/L KT for somatic embryo,and MS＋6-1.0 mg/L BA ＋0.1 mg/L NAA for bud.The rooting media was 1/2 MS＋0.3 mg/L NAA.Active carbon went against the rooting culture of S.japonica.Conclusion The seed germination rate relates to seed quality of S.japonica and has little correlation with the kinds of media.Cotyledon was most suitable for inducing callus.