化学发光成像分析法检测健康食品中的褪黑激素

Chemiluminescence Imaging Determination of Melatonin in Healthy Food

基于在碱性介质中,褪黑激素对H2O2与MoO42-反应产生的微弱化学发光信号有较强的增敏作用,利用高灵敏度、高分辨率的成像分析仪检测发光信号,建立了一种简单、高通量的检测褪黑激素的分析方法并将该方法用于健康食品脑白金中褪黑激素含量的测定。

A simple and high throughput chemiluminescence （CL）imaging method for the determination of melatonin is reported. It is based on the observation that melatonin can greatly enhance the ultra-weak CL between H2O2 and MoO4^2- in alkaline solution, the CL signal can be detected by CL imaging system with high sensitivity and resolution. As a simple application, this, method has been successfully applied into the determination of melatonin in healthy foods.