不同球囊扩张时间转移基因效率的对比研究

Study on the efficiencies of gene transfer with different durations of balloon inflation

为了对不同球囊扩张时间的转移基因效率进行比较。方法：用左旋多聚赖氨酸包被导管球囊，然后吸附携带报告基因Ｌａｃｚ的腺病毒载体（Ａｄ５／ＬａｃＺ），向兔腹主动脉下段定位转移基因。球囊扩张时间分为５分钟、１５分钟和３０分钟。用定性（Ｘ－Ｇａｌ染色）和定量（β－Ｇａｌ活性测定）方法检测基因转移效率。结果：５分钟、１５分钟和３０分钟的基因转移效率无明显差异，十分接近。结论：利用该局部基因转移系统，球囊扩张５分钟即能实现有效的在体。

Objective: The goal of this study was to compare the efficiencies of in vivo gene transfer with different inflation durations of the catheter balloon. Methods: The reporter gene LacZ carried in adenoviral vectors was locally transferred into the lower parts of rabbit abdominal aortic arteries with poly L lysine coated balloon catheter, which was inflated for 5,15 or 30 minutcs, and the efficiencies of gene transfer were qualitatively and quantitatively evaluated with X Gal staining and β Gal activity assay, respectively. Results: The efficiencies of gene transfer with the durations of 5,15 or 30 minutes of balloon inflation showed no significant difference. Conclusion: With the local gene delivery system, local in vivo gene transfer could be effectively achieved with only 5 minutes duration of balloon inflation.