NO及NO合成酶抑制剂对急性肾衰大鼠内源性内皮素分泌的影响

Effects of NO and NO synthetase inhibitor on endogenous endothelin secretion in acute renal failure in rats

在甘油致大鼠ARF模型上,分别观察了NO前体L—Arg及NO合成酶抑制剂L—NNA对ARF大鼠肾功能及内源性ET分泌的影响。结果显示:ARF大鼠ET分泌增多,NO合成减少,L—NNA明显增加ARF大鼠P_(ET)水平(P<0.001),肾小管ET免疫反应阳性颗粒明显增多(P<0.001),肾功能更加恶化。L—Arg则使ARF大鼠P_(ET)水平明显降低(P<0.001),肾小管ET免疫反应阳性颗粒明显减少(P<0.001),肾功能得到改善。提示甘油所致ARF时,NO改善肾功能的作用可能与具抑制内源性ET分泌有关。

The effects of L-arginine (L-Arg), the physiological NO precureor and NO synthetase inhibitor NG-nitro-L-arginine (L-NNA) on endogenous endothelin (ET) secretion and renal function were observed in a model of glycerol-induced acute renal failure (ARF) in rat. It was found that endogenous ET secretion was significantly increased, while serum NO was markedly decreased in ARF rats. The administration of L-NNA to the ARF rats induced significant increases in plasma ET and positive immunoreactive particles of ET in renal tubular epithelial cells(EP cells), and the impairment of renal function was exaggerated. L-Arg might effectively decrease the level of plasma ET and the positive immunoreactive particles of ET in renal tubular EP cells, suggesting the improvement of the renal function. It is suggested that the ability of NO in improving renal function may relatedto the inhibition of endogenous ET secretion in glycerol-induced ARF rats.