哮喘患儿淋巴细胞CD23表达和血清IgE水平相互关系的研究

The role of CD23 expression on lymphocytes and serum IgE levels in immunological pathogenesis of asthma in children

本文应用单克隆抗体结合APAAP技术,Dot—blot地高辛标记核酸杂交技术,研究了哮喘患儿淋巴细胞CD23mRNA和膜CD23分子表达,同时检测了血清总IgE水平。结果显示:哮喘患者淋巴细胞CD23mRNA表达增高,而正常对照组未见杂交信号;外周血单个核细胞和B细胞CD23分子表达均明显增加,与对照组相比尸值分别<0.01,<0.001以B细胞CD23分子表达增加更显著。血清IgE水平异常升高,B细胞CD23分子表达与血清IgE水平之间呈显著正相关。结果提示CD23直接参与了过敏性哮喘的发病。

The expression of lymphocyte CD23 mRNA and CD23 molecules in children with asthma and health controls were studied. Serum IgE levels were also detected. The resuits showed: (1) High expression of lymphocyte CD23 mRNA in asthmatic children, while no expression is observed in normal controls. (2) Markedly increased expression of CD23 molecules in peripheral blood monocytes and B cells in asthmatic children, versus healthy controls (P<0.01, P<0.001 respectively). (3) Markedly elevated serum IgE levels in asthmatic children compared with controls; higher IgE levels in children with extrinsic asthma compared to those with intrinsic asthma (P<0.01). (4) Positive linear correlation between CD23 molecules expression and serum IgE levels in asthmatic children (r=0.679, P<0.01). These results suggested that CD23 may play a critical role in immunologic pathogenesis of asthma and it may be involved in the regulation of IgE synthesis.