维生素C对氟化钠和化合物48-80诱导大鼠肥大细胞释放组胺的影响

Effects of vitamin C on histamine release induced by sodium fluoride and compound 48 80 from isolated mast cells 1

氟化钠（ＮａＦ）和化合物４８－８０均可诱发大鼠胸腔，腹腔肥大细胞释放组胺，前者依赖细胞外钙参与，后者与细胞外钙关系不大，二者的最适诱导浓度分别为１０ｍｍｏｌＬ－１和０．２ｍｇＬ－１．维生素Ｃ可促进ＮａＦ诱导的组胺释放，但对化合物４８－８０所诱导的组胺释放无明显影响．将肥大细胞置于３７℃，２ｈ，ＮａＦ或化合物４８－８０诱导的组胺释放率均下降．预先加入维生素Ｃ１ｍｍｏｌＬ－１，此现象显著减弱．由此提示：ＮａＦ激发肥大细胞，增敏其对Ｃａ２＋分泌作用不是由自由基引发的，另一方面也不排除３７℃长时间温育细胞产生自由基可能与降低细胞对ＮａＦ－Ｃａ２＋系统和化合物４８－８０的敏感性有关．

Sodium fluoride (NaF) and compound 48 80, the optimum concentration of which were 10 mmol·L 1 and 0.2 mg·L 1 respectively, could induce the release of histamine from isolated rat thoracic and peritoneal mast cells (CPMC). Vitamin C could promote histamine release induced by NaF on incubated rat CPMC, but, had no significant effect on the release of histamine induced by compound 48 80. CPMC preincubated at 37 ℃ for 2 h could reduce the histamine release induced by NaF or compound 48 80. However, it could be counteracted by vitamine C pretreatment. The results suggest that NaF stimulating mast cells and increasing the secretary of calcium are not generated by free radicals. On the other hand, it can not exclude that free radicals generated from CPMC preincubated at 37 ℃ for 2 h decrease the sensitivity of cells to NaF Ca 2+ and compound 48 80.