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爪哇稻Wanilava细胞质雄性不育系的创建及AFLP指纹图谱分析 被引量:8

Breeding of Cytoplasmic Male Sterile Line Derived from Oryza sativa subsp.javanica cv.Wanilava and Its AFLP Fingerprints
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摘要 初步鉴定了爪哇稻Wanilava细胞质雄性不育系的特征特性,并以2套水稻(Oryza sativa)同核异质材料273JW(爪哇型胞质不育系)、273D(D型胞质不育系)及其对应的保持系273B,803JW(爪哇型胞质不育系)、803WA(野败型胞质不育系)及其对应的保持系803B为研究对象,利用AFLP分子标记技术构建了JW型不育胞质的指纹图谱,分离了JW型胞质与野败型胞质及保持系的差异片段。 The characteristics of a novel cytoplasmic male sterile (CMS) line derived from Oryza sativa subsp, javanica cv. Wanilava (denominated as JW type) were analyzed. Two sets of isonucleusalloeytoplasmic lines (273D, 273JW and 273B; 803WA, 803JW and 803B, respectively) were bred by advanced backcrosses using the maintainer lines (273B and 803B, respectively) as recurrent parents, each containing individual cognate nucleus with different cytoplasms. Considerable genetic diversities between (7MS JW (CMS from javanica cv. Wanilava) and CMS-WA (CMS of wild abortive from O. rufipogon) or CMS D (CMS from African indica cv. Dissi) were revealed by AFI.P. The differential fragments from the cytoplasms were isolated.
出处 《中国水稻科学》 CAS CSCD 北大核心 2008年第1期28-32,共5页 Chinese Journal of Rice Science
基金 四川省“十一五”水稻育种攻关资助项目(2006-YZGG-1)
关键词 细胞质雄性不育 同核异质材料 扩增片段长度多态性 指纹图谱 爪哇稻 cytoplasmic male sterility isonueleus allocytoplasmic lines amplified fragment length polymorphism fingerprint javanica rice
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