NaCl处理下两种补血草种子萌发和幼苗抗性的比较

Comparison of Seed Germination and Resistance in Two Limonium Seedlings under NaCl Treatment

用不同浓度的NaCl处理盐生植物黄花补血草和大叶补血草,研究其种子萌发和幼苗叶片中叶绿素和可溶性蛋白等各项生理指标的变化,分析比较这两种植物的耐盐性。结果表明,NaCl处理抑制两种补血草种子的萌发,此效应具有浓度依赖性,低浓度的NaCl促进黄花补血草根和大叶补血草茎叶的生长,高浓度的NaCl抑制两种补血草幼苗的生长;与对照比,50、150和300 mmol.L-1NaCl处理后,两种补血草幼苗叶片的叶绿素a(Chla)、叶绿素b(Chlb)和叶绿素总量均减少,而H2O2和MDA含量却增加;低浓度的NaCl处理使两种补血草幼苗叶片可溶性蛋白含量增加,而高浓度的NaCl处理使其可溶性蛋白的含量降低。此外,相同浓度的NaCl处理对大叶补血草种子萌发、叶绿素含量等指标的抑制作用明显强于黄花补血草,且其MDA产生明显强于黄花补血草,这些表明黄花补血草可能比大叶补血草具有较强的耐盐性。

The changes of physiological indexes and seed germination were investigated in two halophytes, Limonium aureum （ Linn. ） Hill （Huanghua） and L. gmelinii （ Willd. ） Kuntze （Daye） treated with different NaCl concentration. The results showed that the germination of two L. sinuatum seeds was inhibited by NaCl in concentration-dependent manner. Compared to the control, there was decrease of chlorophyll a, chlorophyll b and total chlorophyll content in Huanghua and Daye seedling leaves after 50, 150, 300 mmol·L^-1 NaCl treatment, and increase in the amount of H2O2 and MDA. Soluble protein content was enhanced in two L. sinuatum leaves when exposed to lower NaCl concentration, but decreased to higher NaCl concentration. In addition, the inhibition of the seed germination and chlorophyll content induced by NaCl in Daye was stronger than in Huanghua, suggesting that the salt tolerance of Huanghua was higher than that of Daye.