螺旋藻多糖对肿瘤细胞生长及Hela细胞早期凋亡作用的实验研究

Experimental studies on antiproliferation activities and early apoptosis inducement of polysaccharides from Spirulina platensis

采用MTT法(四甲基偶氮唑盐比色法)研究了钝顶螺旋藻多糖(PSP)对Hela细胞及HepG2细胞生长的抑制作用。结果表明,随PSP浓度及培养时间的增加,肿瘤细胞存活率逐渐降低,抑制率逐渐增加,以PSP40 mg/L作用72 h最为显著。应用Annexin V/PI双染色流式细胞仪检测了早期Hela细胞凋亡,未经PSP处理的正常Hela细胞凋亡细胞极少,经PSP处理的细胞,凋亡细胞的百分比明显高于正常对照组,其作用随着剂量的增加和时间的延长而增强,具有量效关系和时效关系。结果说明PSP的抗肿瘤机制,除了诱导肿瘤细胞凋亡,还存在细胞毒性等其他机制,是多种机制共同作用的结果。

In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis （PSP） were evaluated on Hela cells and HepG2 cells by MTT assay , the results showed that PSP （2.5-40 mg/L, 48-72 h） exhibited dose and time inhibition effects on the cell growth. The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining, the apoptosis rates induced by PSP （5,10,20,40 mg/L ） were 30.3%, 32. 6%, 34. 8% and 38. 8% respectively, which were higher than those of the normal control group（P〈0.05）. Meanwhile, the apoptosis rate treated by PSP（40 rag/L） also increased in time-dependent manner. These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis.