转移生长因子β_1在牛眼小梁网的表达

Expression of messenger RNA for transforming growth factor β 1 in bovine trabecular meshwork

目的研究转移生长因子β１（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒβ１，ＴＧＦ－β１）与开角型青光眼的关系。方法用异硫氰酸胍法提取２４只新生牛眼小梁网的总ＲＮＡ，将ＴＧＦ－β３３质粒导入大肠杆菌ＨＢ１０１中充分扩增、ＢａｍＨＩ酶切后，片段以α－３２－Ｐ－ｄＡＴＰ标记成ｃＤＮＡ（ｃｏｍｐｌｅｍｅｎｔａｒｙＤＮＡ）探针，ＲＮＡ斑点杂交，放射自显影法观察和测定牛眼小梁网的ＴＧＦ－β１ｍＲＮＡ的表达。结果牛眼小梁网中有ＴＧＦ－β１ｍＲＮＡ的表达。结论房水中的部分ＴＧＦ－β１由小梁细胞分泌。此结果可以作为利用分子生物学手段研究ＴＧＦ－β１ｍＲＮＡ的异常合成、活化、清除及与开角型青光眼病理改变间关系的基础。

Objective To investigate the relationship between transforming growth factor β 1(TGF β 1) and primary open angle glaucoma. Methods Total RNA of 24 new born bovine trabecular tissue was extracted by guanidine isothiocyanate method. The TGF β 33 plasmid was brought into colibacillus HB101 and amplified. After Bam HI enzyme degradation and label with α 32 P dATP, the RNA was hybridized with the complementary DNA (cDNA) probe and examined by autoradiography to determine the expression of messenger RNA (mRNA) for TGF β 1. Results The presence of mRNA for TGF β 1 in bovine trabecular meshwork was confirmed. Conclusion The TGF β 1 present in normal aqueous humor at least partly is derived from the trabecular meshwork. The result offers a basis for understanding the relationship between abnormal synthesis, activation and clearance of TGF β 1 and the pathogenesis of primary open angle glaucoma in molecular biology.